Leptin and connective tissue growth factor in advanced glycation end-product-induced effects in NRK-49F cells

Journal of Cellular Biochemistry
Chu-I LeeLea-Yea Chuang

Abstract

Previously, we showed that Janus kinase 2 (JAK2) is important in advanced glycation end-product (AGE)-induced effects in renal interstitial (NRK-49F) fibroblasts. Leptin is a JAK2-activating cytokine via the long form leptin receptor (Ob-Rb). Leptin and connective tissue growth factor (CTGF) may be involved in renal fibrosis. However, the relationship between leptin and CTGF in terms of AGE-induced effects remains unknown. Thus, the effects of AGE (150 microg/ml) and leptin on mitogenesis, CTGF and collagen expression in NRK-49F cells were determined. We found that leptin and AGE increased mitogenesis and type I collagen protein expression at 3 and 7 days, respectively. AGE increased leptin mRNA and protein expression at 2-3 days. AGE increased CTGF mRNA and protein expression at 3-5 days. AG-490 (JAK2 inhibitor) abrogated AGE-induced leptin mRNA and protein expression at 2-3 days. AG-490 and Ob-Rb anti-sense oligodeoxynucleotides (ODN) abrogated AGE-induced CTGF mRNA and protein expression at 3-5 days. AG-490 and CTGF anti-sense ODN abrogated AGE-induced mitogenesis and collagen protein expression at 7 days. Additionally, leptin dose (0.2-1 microg/ml) and time (1-2 days)-dependently increased CTGF protein expression. AG-490 ab...Continue Reading

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Citations

Jun 2, 2011·Journal of Cell Communication and Signaling·Xiaoyu WangStephen M Twigg
Nov 3, 2007·Liver International : Official Journal of the International Association for the Study of the Liver·Caroline HoraUNKNOWN Swiss Hepatitis C Cohort Study Group
Sep 18, 2015·Frontiers in Medicine·Henricus A M MutsaersPeter Olinga
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Jun 25, 2021·Nutrition·Temitope Gabriel Adedeji, Emiola Oluwabunmi Olapade-Olaopa

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