Leptin and leptin receptor genes in tongue sole (Cynoglossus semilaevis): Molecular cloning, tissue distribution and differential regulation of these genes by sex steroids

Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology
Yongjiang XuKangli Ren

Abstract

Leptin (Lep) is a key factor for the regulation of food intake and energy homeostasis in mammals. To date, a number of studies have provided evidence for the existence of multiple leptin genes in teleosts, but not much information is available in fish regarding the regulation of leptin genes by sex steriods. As a first step, two leptin genes (lepa and lepb) and a leptin receptor (lepr) gene were cloned from the half-smooth tongue sole (Cynoglossus semilaevis), a representative species of the order Pleuronectiformes. The full-length cDNAs of tongue sole lepa and lepb were 1265 bp and 1157 bp in length, encoding for proteins of 160 aa and 158 aa, respectively. The three-dimensional structures modeling of tongue sole LepA and LepB showed strong conservation of tertiary structure with other vertebrates. The full-length cDNA of tongue sole lepr was 4576 bp, encoding a protein of 1133 aa which contained all functionally important domains conserved among vertebrate LepRs. Tissue distribution analysis showed that tongue sole lepa mRNA was highly detectable in the ovary and brain, while lepb mRNA was ubiquitously expressed in various tissues. Notably, the tongue sole lepr mRNA was most abundant in the ovary. Using a primary hepatocyte c...Continue Reading

Citations

Feb 16, 2021·Molecular and Cellular Endocrinology·Ayelén Melisa Blanco, José Luis Soengas
Jul 25, 2021·International Journal of Molecular Sciences·Jamie L Mankiewicz, Beth M Cleveland
Aug 4, 2021·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Amrita BakshiUmesh Rai

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