Leptin improves membrane fluidity of erythrocytes in humans via a nitric oxide-dependent mechanism--an electron paramagnetic resonance investigation

Biochemical and Biophysical Research Communications
K TsudaI Nishio

Abstract

Abnormalities in physical properties of the cell membranes may underlie the defects that are strongly linked to hypertension, stroke, and other cardiovascular diseases. Recently, there has been an indication that leptin, the product of the human obesity gene, actively participates not only in the metabolic regulations but also in the control of cardiovascular functions. In the present study, to assess the role of leptin in the regulation of membrane properties, the effects of leptin on membrane fluidity of erythrocytes in humans are examined. The membrane fluidity of erythrocytes in healthy volunteers by means of an electron paramagnetic resonance (EPR) and spin-labeling method is determined. In an in vitro study, leptin decreased the order parameter (S) for 5-nitroxide stearate (5-NS) and the peak height ratio (ho/h-1) for 16-NS obtained from EPR spectra of erythrocyte membranes in a dose-dependent manner in healthy volunteers. The finding indicated that leptin increased the membrane fluidity and improved the microviscosity of erythrocytes. The effect of leptin on the membrane fluidity was significantly potentiated by the nitric oxide (NO) donors, L-arginine and S-nitroso-N-acetylpenicillamine (SNAP), and a cyclic guanosine mo...Continue Reading

References

Feb 1, 1978·The Journal of Clinical Investigation·B SatoF H Tyler
Aug 18, 1977·The New England Journal of Medicine·R A Cooper
Aug 1, 1991·Hypertension·S T Turner, V V Michels
Jan 1, 1980·Clinical and Experimental Hypertension : CHE·Y YamoriA Ooshima
Apr 3, 1980·The New England Journal of Medicine·M CanessaD C Tosteson
Sep 23, 1994·Cell·C Nathan, Q W Xie
Dec 10, 1996·Proceedings of the National Academy of Sciences of the United States of America·T GainsfordD J Hilton
Jul 15, 1997·The Journal of Clinical Investigation·W G HaynesW I Sivitz
Jan 1, 1998·The New England Journal of Medicine·J StevensJ L Wood
Feb 7, 1998·Hypertension·E W ShekJ E Hall
Feb 9, 2000·Diabetologia·G Frühbeck, J Salvador
Jun 30, 2000·Biochemical and Biophysical Research Communications·K KimuraI Nishio
Oct 18, 2001·Molecular and Cellular Endocrinology·A Konstantinou-TegouG Koliakos
Jan 5, 2007·Behavioral Neuroscience·April E RoncaJeffrey R Alberts

❮ Previous
Next ❯

Citations

Sep 2, 2004·Obesity Research·Kazushi Tsuda, Ichiro Nishio
Jul 20, 2004·Journal of Internal Medicine·S SöderbergT Olsson
Aug 13, 2010·Diabetes Research and Clinical Practice·D Simmons
Mar 31, 2016·Journal of Cellular Physiology·Aurore CollinMarie-Chantal Farges
Feb 11, 2004·Hypertension·Kazushi Tsuda, Ichiro Nishio
Jun 9, 2004·Circulation·Kazushi Tsuda, Ichiro Nishio
Sep 29, 2004·Circulation·Kazushi Tsuda, Ichiro Nishio
Dec 8, 2007·Stroke; a Journal of Cerebral Circulation·Kazushi Tsuda
Mar 28, 2009·Stroke; a Journal of Cerebral Circulation·Kazushi Tsuda
Jan 19, 2019·British Journal of Pharmacology·Ijeoma E ObiJennifer S Pollock
Aug 31, 2011·Critical Reviews in Clinical Laboratory Sciences·Ivan Spasojević
Jan 15, 2005·American Journal of Physiology. Heart and Circulatory Physiology·Silvia Bertuglia, Andrea Giusti
Sep 11, 2019·Nutrients·Sara BecerrilGema Frühbeck
Nov 29, 2005·Journal of Chemical Information and Modeling·Ivan SpasojevićGoran Bacić

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cardiovascular Disease Pathophysiology

Cardiovascular disease involves several different processes that contribute to the pathological mechanism, including hyperglycemia, inflammation, atherosclerosis, hypertension and more. Vasculature stability plays a critical role in the development of the disease. Discover the latest research on cardiovascular disease pathophysiology here.