PMID: 11919545Mar 29, 2002Paper

Leptin in anorexia nervosa and bulimia nervosa: importance of assay technique and method of interpretation

The Journal of Laboratory and Clinical Medicine
Robert FrederichClaire Pomeroy

Abstract

Studies of the role of leptin in patients with anorexia nervosa and bulimia nervosa have conflicted in their data and interpretation. Such differences may be a result of the assay methods used or the way results are compared with those from normal controls. To investigate these possibilities, we analyzed serum leptin levels in anorexic, bulimic, obese, and control individuals, thereby spanning the full range of human body weights, using three frequently employed commercial kits. Kits from Linco (St Louis, MO) and DSL (Webster, TX) employ a radioimmunoassay method, and the R&D Systems kit (Minneapolis, MN) uses an enzyme-linked immunosorbent assay. We found that the three kits provide results that are highly linearly correlated with each other and remarkably linearly related to percent ideal body weight (%IBW) over more than three orders of magnitude (Linco, r = 0.90; R&D, r = 0.87; DSL, r = 0.86). For very low leptin levels, the more sensitive kits from R&D and Linco appeared to give more reliable results. Measurement method does not appear to explain the literature conflicts. We found that patients with anorexia nervosa have serum leptin values that lie above the line extrapolated from the %IBW/leptin curve generated from anal...Continue Reading

References

Feb 1, 1996·The New England Journal of Medicine·R V ConsidineT L Bauer
Apr 1, 1997·International Journal of Obesity and Related Metabolic Disorders : Journal of the International Association for the Study of Obesity·A K TurpeinenM I Uusitupa
Jul 1, 1997·International Journal of Obesity and Related Metabolic Disorders : Journal of the International Association for the Study of Obesity·H ShimizuM Mori
Jan 24, 1998·Metabolism: Clinical and Experimental·B BaranowskaK Roguski
Jan 24, 1998·Metabolism: Clinical and Experimental·H M PerryG Wittert
Apr 30, 1998·The International Journal of Eating Disorders·S HerpertzJ Hebebrand
Jun 3, 1998·American Journal of Obstetrics and Gynecology·T J HighmanP M Catalano
Jun 26, 1998·Clinical Endocrinology·J H PinkneyV Mohamed-Ali
Aug 26, 1998·Clinical Endocrinology·R K StøvingC Hagen
Nov 21, 1998·International Journal of Obesity and Related Metabolic Disorders : Journal of the International Association for the Study of Obesity·A F HeiniR L Weinsier
Nov 21, 1998·European Journal of Clinical Investigation·H A KoistinenV A Koivisto
Dec 19, 1998·Molecular Psychiatry·L AudiA Carrascosa
Feb 19, 1999·Metabolism: Clinical and Experimental·Y NakaiF Kurimoto
Apr 8, 1999·International Journal of Obesity and Related Metabolic Disorders : Journal of the International Association for the Study of Obesity·S C HoA C Fok
May 20, 1999·Hormone and Metabolic Research = Hormon- Und Stoffwechselforschung = Hormones Et Métabolisme·K MathiakW Köpp
Jun 1, 1999·The International Journal of Eating Disorders·Y NakaiF Kurimoto
Jun 16, 1999·Gynecological Endocrinology : the Official Journal of the International Society of Gynecological Endocrinology·R M CentoA Lanzone
Jul 1, 1999·Clinical and Experimental Pharmacology & Physiology·T OkazakiM Ikeda
Jul 27, 1999·Biological Psychiatry·K A GendallM C La Via
Jul 28, 1999·The International Journal of Eating Disorders·P S MehlerW T Donahoo
Aug 11, 1999·The International Journal of Eating Disorders·S A LearC L Birmingham
Nov 7, 1999·International Journal of Sports Medicine·N MatejekH Böhles
Mar 4, 2000·Journal of Psychiatric Research·J FreyB M Herpertz-Dahlmann
Jun 6, 2000·The American Journal of Clinical Nutrition·A PolitoD Giannini
Aug 12, 2000·Psychoneuroendocrinology·T D BrewertonW T Garvey
Aug 15, 2000·Nutrition Reviews·J C Fleet

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Citations

Jan 8, 2008·The American Journal of Clinical Nutrition·Anja W DynesenBirgitte Nauntofte
Mar 11, 2005·Expert Opinion on Therapeutic Targets·James L RoerigKristine J Steffen
Jul 1, 2015·The Journal of Physiological Sciences : JPS·Mitsuhiro YoshimuraYoichi Ueta
Apr 17, 2004·Nutritional Neuroscience·Agnes K Ayton
Nov 6, 2009·Helicobacter·Lisette G CapelleErnst J Kuipers

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