Abstract
To test the hypothesis that leptin secreted from adipose tissue is a mediator linking obesity and pancreatic islet hypertrophy, we examined the effects of leptin on proliferative and apoptotic responses in rat islet cells. Rat pancreatic islets were isolated and incubated with 0, 1, 5, or 75 nM leptin for 24 h under serum-deprived conditions. Cell viability was assessed with 2,5-diphenyltetrazolium bromide and trypan blue dye exclusion tests. Cell proliferation and apoptosis were evaluated with 5-bromo-2'-deoxyuridine incorporation into DNA and DNA ladder formation, respectively. Incubation for 24 h with 1 and 5 nM leptin, the concentrations observed in obese subjects, increased the viability of isolated pancreatic islet cells. Five nanomolar concentrations of leptin did not stimulate 5-bromo-2'-deoxyuridine incorporation into incubated islet cells, indicating no influence on cell proliferation, but did inhibit DNA ladder formation, a hallmark of cell apoptosis. Moreover, 5 nM leptin reduced the triglyceride content and suppressed inducible nitric oxide synthase mRNA expression in incubated islets. These results suggest that leptin increased viable cell numbers via suppression of apoptosis in isolated pancreatic islet cells und...Continue Reading
References
Nov 1, 1993·Biotechnology Progress·S A AltmanG Rao
Apr 29, 1997·Proceedings of the National Academy of Sciences of the United States of America·M ShimabukuroR H Unger
Jul 15, 1997·The Journal of Clinical Investigation·M ShimabukuroR H Unger
Oct 6, 1997·The Journal of Clinical Investigation·M ShimabukuroR H Unger
Oct 10, 1997·Endocrinology·Y TanizawaY Oka
Jan 22, 1998·Biochemical and Biophysical Research Communications·K TanabeY Oka
Feb 17, 1998·European Journal of Immunology·H U SimonK Blaser
Apr 16, 1998·Proceedings of the National Academy of Sciences of the United States of America·M ShimabukuroR H Unger
Mar 31, 1998·Diabetes·A PickK S Polonsky
Aug 5, 1998·Proceedings of the National Academy of Sciences of the United States of America·M ShimabukuroR H Unger
May 18, 1999·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·J P MacManusU Tuor
Dec 14, 1999·Biological Chemistry·T BittorfJ Brock
Feb 9, 2000·Diabetologia·G Frühbeck, J Salvador
Nov 28, 2000·International Journal of Obesity and Related Metabolic Disorders : Journal of the International Association for the Study of Obesity·M S IslamV Emilsson
Citations
May 14, 2004·Proceedings of the National Academy of Sciences of the United States of America·Kathrin MaedlerMarc Y Donath
Dec 14, 2006·American Journal of Physiology. Endocrinology and Metabolism·S M TurnerM K Hellerstein
May 12, 2012·International Journal of Endocrinology·Elena TarabraMichael Khan
Mar 7, 2012·International Journal of Nanomedicine·Sunil K SinghDebabrata Dash
Nov 17, 2015·Metabolic Syndrome and Related Disorders·Prasenjit Manna, Sushil K Jain
Jun 3, 2004·Journal of Cellular Physiology·Hongxiang HuiRiccardo Perfetti
Jan 11, 2007·The British Journal of Surgery·M R HodaK E Barrett
Mar 21, 2007·Diabetologia·A OgeM E Patti
Aug 3, 2007·Journal of Gastroenterology·Sevim ErcanAydan Babül
Oct 16, 2007·Molecular Reproduction and Development·Muren HerridJames R McFarlane
Mar 18, 2008·BJU International·M Raschid Hoda, Gralf Popken
Oct 3, 2009·The Anatomical Record : Advances in Integrative Anatomy and Evolutionary Biology·Bernard G SlavinJanis S Fisler
Aug 21, 2010·Diabetic Medicine : a Journal of the British Diabetic Association·B ThorandW Koenig
Sep 23, 2010·Journal of Gastrointestinal Surgery : Official Journal of the Society for Surgery of the Alimentary Tract·Patrick B WhiteNicholas J Zyromski
Aug 20, 2005·Journal of Cellular Physiology·Cecilia Garofalo, Eva Surmacz
Feb 28, 2007·International Journal of Obesity : Journal of the International Association for the Study of Obesity·S SöderbergJ E Shaw
Dec 3, 2015·American Journal of Physiology. Endocrinology and Metabolism·Thierry NtimbaneEmile Levy
Nov 27, 2019·Clinical Science·Nicolás Gómez-Banoy, James C Lo
Jun 2, 2020·Nutrition and Cancer·Gulsah Ozcan-SınırOmer Utku Copur
Jun 26, 2003·Clinical and Experimental Immunology·V Sánchez-MargaletC Gonzalez-Yanes
Feb 24, 2007·Diabetes/metabolism Research and Reviews·James E P Brown, Simon J Dunmore
Aug 11, 2010·Autoimmunity·Annie J KrugerRita Bortell
Oct 13, 2011·Pancreas·Mika HoriMami Takahashi
May 4, 2021·Frontiers in Pharmacology·Min-Hyun Kim, Hyeyoung Kim
Mar 27, 2003·Cellular Immunology·Souad Najib, Víctor Sánchez-Margalet