Leptin induces proliferation of pancreatic beta cell line MIN6 through activation of mitogen-activated protein kinase

Biochemical and Biophysical Research Communications
K TanabeY Oka

Abstract

Leptin at 1-5 nM, the concentrations observed in obese subjects, caused an increase in the active form of mitogen-activated protein kinase (MAPK) that was accompanied by increased tyrosine phosphorylation of STAT-1 and STAT-3 in a mouse pancreatic beta cell line, MIN6. Leptin also increased DNA synthesis and cell viability in MIN6 cells based on the results of [3H]-thymidine incorporation and colorimetric MTT assay, respectively. The specific MAPK-inhibitor PD98059 blocked not only the MAPK activation but also the increment in DNA synthesis and cell viability caused by leptin. Thus, leptin stimulates both the MAPK and the Janus kinase (JAK)-STAT cascade as well as inducing proliferation through the MAPK cascade in MIN6 cells. This mechanism might account, at least in part, for obesity-induced pancreatic islet hypertrophy.

References

Jul 28, 1995·Science·M A PelleymounterF Collins
Aug 15, 1995·Proceedings of the National Academy of Sciences of the United States of America·D T DudleyA R Saltiel
Feb 1, 1996·The New England Journal of Medicine·R V ConsidineT L Bauer
Jul 16, 1996·Biochemical and Biophysical Research Communications·T J KiefferJ F Habener
Aug 6, 1996·Proceedings of the National Academy of Sciences of the United States of America·H BaumannL A Tartaglia
Jul 1, 1996·Endocrinology·I A BarashR A Steiner
Dec 10, 1996·Proceedings of the National Academy of Sciences of the United States of America·T GainsfordD J Hilton
Apr 18, 1997·The Journal of Biological Chemistry·G MüllerG Preibisch
May 16, 1997·The Journal of Biological Chemistry·Y TakahashiK Chihara
May 27, 1997·Proceedings of the National Academy of Sciences of the United States of America·S Khoo, M H Cobb
Oct 23, 1997·Biochemical and Biophysical Research Communications·M S IslamV Emilsson

❮ Previous
Next ❯

Citations

Apr 3, 2001·European Journal of Clinical Investigation·A Glasow, S R Bornstein
Oct 31, 2002·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Moshe MarikovskyMiriam Friedman-Einat
Nov 23, 2006·Infection and Immunity·Mahalia S DesruisseauxPhilipp E Scherer
Aug 15, 2000·Annual Review of Nutrition·R B Harris
Dec 14, 2006·American Journal of Physiology. Endocrinology and Metabolism·S M TurnerM K Hellerstein
May 12, 2012·International Journal of Endocrinology·Elena TarabraMichael Khan
Aug 23, 2000·The Journal of Clinical Investigation·S FrankJ Pfeilschifter
May 25, 2007·BMC Molecular Biology·Paul HekermanWalter Becker
Jan 8, 2008·Bioscience, Biotechnology, and Biochemistry·Taiyong YuGongshe Yang
Mar 27, 2012·Journal of Molecular Endocrinology·Laura MarroquíIvan Quesada
Sep 20, 2012·The Journal of Endocrinology·Simon J Dunmore, James E P Brown
Jun 19, 2001·Proceedings of the National Academy of Sciences of the United States of America·S N FlierC R Kahn
May 14, 2004·Proceedings of the National Academy of Sciences of the United States of America·Kathrin MaedlerMarc Y Donath
Oct 2, 2015·International Journal of Molecular Sciences·Adrián González-AlonsoJosé L Quiles
Apr 18, 2012·Expert Opinion on Biological Therapy·Juan Domínguez-BendalaCamillo Ricordi
Jul 22, 2004·Journal of Veterinary Medicine. A, Physiology, Pathology, Clinical Medicine·M MácajováM Zeman
Jun 26, 2003·Clinical and Experimental Immunology·V Sánchez-MargaletC Gonzalez-Yanes
Feb 1, 2008·Clinical and Experimental Immunology·P Fernández-RiejosV Sánchez-Margalet
Dec 8, 2006·Annals of the New York Academy of Sciences·Krystallenia AlexandrakiAnastasios Kalofoutis
Jul 10, 2001·The Journal of Investigative Dermatology·B StallmeyerS Frank
Jul 18, 2014·PloS One·Hirochika UmekiKazuhito Satomura
Mar 24, 2015·PloS One·Susumu TadokoroKazuhito Satomura
Jan 31, 2006·Molecular and Cellular Endocrinology·Ian L P Beales, O Ogunwobi
May 2, 2017·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Eliane Macedo Sobrinho SantosLucyana Conceição Farias
Apr 1, 2008·Acta Cirúrgica Brasileira·Ioannis LiapakiConstantinos Simopoulos
Oct 12, 2017·Reproduction : the Official Journal of the Society for the Study of Fertility·Malena SchantonCecilia L Varone
Dec 31, 2004·Gynecological Endocrinology : the Official Journal of the International Society of Gynecological Endocrinology·I IwamotoT Douchi
Aug 2, 2003·Circulation Research·Venkatesh RajapurohitamMorris Karmazyn
Mar 17, 2009·Pancreas·Maria Fernanda R GracianoAngelo R Carpinelli
Nov 28, 2000·International Journal of Obesity and Related Metabolic Disorders : Journal of the International Association for the Study of Obesity·M S IslamV Emilsson
Apr 17, 2002·Endocrinology·Kazuhiro KawamuraToshinobu Tanaka
Sep 11, 2008·American Journal of Physiology. Endocrinology and Metabolism·L AttigL Abdennebi-Najar
Jun 26, 2003·Annals of Medicine·Jenni Harvey
Oct 14, 2004·American Journal of Physiology. Endocrinology and Metabolism·Melanie J LutherPeter M Jones
Jan 19, 2012·Journal of Cellular Biochemistry·Moria ChetbounTovit Rosenzweig
Jun 15, 2011·Journal of Huazhong University of Science and Technology. Medical Sciences = Hua Zhong Ke Ji Da Xue Xue Bao. Yi Xue Ying De Wen Ban = Huazhong Keji Daxue Xuebao. Yixue Yingdewen Ban·Yi LiuHui Sheng
Jan 25, 2000·American Journal of Physiology. Endocrinology and Metabolism·T J Kieffer, J F Habener

❮ Previous
Next ❯

Related Concepts

Related Feeds

© 2021 Meta ULC. All rights reserved