Leptin inhibits glucose intestinal absorption via PKC, p38MAPK, PI3K and MEK/ERK

PloS One
Ola El-Zein, Sawsan I Kreydiyyeh

Abstract

The role of leptin in controlling food intake and body weight is well recognized, but whether this is achieved by modulating nutrient absorption is still a controversial issue. The aim of this work was to investigate the direct effect of luminal leptin on glucose intestinal absorption and elucidate for the first time its signaling pathway. Fully differentiated Caco-2 cells grown on transwell filters were used for glucose transport studies. Leptin caused a significant reduction in glucose absorption. Individual and simultaneous inhibition of ERK, p38MAPK, PI3K or PKC abrogated completely the inhibitory effect of leptin. Activating PKC, lead to a stimulatory effect that appeared only when ERK, p38MAPK, or PI3K was inactive. Moreover, leptin increased the phosphorylation of ERK, Akt and p38MAPK. This increase changed into a decrease when p38MAPK and PKC were inactivated individually. Inhibiting ERK maintained the leptin-induced up-regulation of p-Akt and p-p38MAPK while inhibiting PI3K reduced the level of p-ERK and p-Akt but maintained the increase in p-p38MAPK. These results suggest that leptin reduces glucose absorption by activating PKC. Although the latter modulates glucose absorption via a stimulatory and an inhibitory pathw...Continue Reading

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Citations

Dec 17, 2014·Cellular Signalling·Ola El-ZeinSawsan Ibrahim Kreydiyyeh
Nov 10, 2015·Laboratory Investigation; a Journal of Technical Methods and Pathology·Jessica A Dominguez RiegTimo Rieg
Mar 7, 2015·Life Sciences·Gabriela Fernández-FormosoSulay Tovar
Dec 19, 2017·Global Heart·Jeffrey I MechanickW Timothy Garvey
Nov 26, 2021·Bioengineered·Ling MeiTao Zhang

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BETA
protein assay
PMA

Software Mentioned

Instat
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Excel
Pro Analyzer

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