PMID: 9426383Jan 14, 1998Paper

Leptin is a physiologically important regulator of food intake

International Journal of Obesity and Related Metabolic Disorders : Journal of the International Association for the Study of Obesity
L BrunnerN Levens

Abstract

These studies were designed to test the hypothesis that endogenous leptin, acting within the brain plays a physiologically important role in the control of food intake in lean rats. Antibodies directed against mouse leptin were raised in rabbits. The purified IgG fractions prepared from pre-immune and immune sera were injected into the right lateral ventricle of lean Sprague-Dawley rats and obese Zucker fatty fa/fa rats. Changes in food intake were measured over the following 20 h period. The anti-leptin antibodies recognized a major epitope in the C-terminal region of the leptin molecule. The antibodies bound both mouse and rat leptin with high affinity, but did not bind human leptin, or a selected range of other hormones and neurotransmitters known to affect food intake. In competition studies, the binding of mouse, but not human leptin to the human Ob-Rb receptor was prevented by the antibodies. This indicates that the antibodies can block the action of leptin by preventing its binding to the ob-Rb receptor. Injection of the anti-leptin antibodies into the brain of lean rats led to an increase in food intake during the first hour after injection which was not compensated during the following 19 h period. Injection of the ant...Continue Reading

Citations

Mar 2, 2002·Domestic Animal Endocrinology·M TaouisA Gertler
Sep 11, 2002·Domestic Animal Endocrinology·S MargeticR A Hill
Feb 28, 2001·Diabetes, Obesity & Metabolism·J M Bryson
Feb 3, 2000·Critical Reviews in Clinical Laboratory Sciences·J Himms-Hagen
Feb 1, 2005·Regulatory Peptides·Vani X OliveiraAntonio Miranda
Oct 19, 2016·The Journal of Endocrinology·Riccardo DoreCarla Schulz
Jan 11, 2000·International Journal of Obesity and Related Metabolic Disorders : Journal of the International Association for the Study of Obesity·A A BurnsI R Rowland
Mar 19, 2005·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Kimberly A SinghJoseph R Vasselli
Apr 25, 2001·International Journal of Obesity and Related Metabolic Disorders : Journal of the International Association for the Study of Obesity·S IossaG Liverini
Sep 6, 2007·American Journal of Physiology. Endocrinology and Metabolism·L PinillaE Aguilar
Nov 24, 1999·Current Opinion in Clinical Nutrition and Metabolic Care·L Brunner, N Levens
Dec 22, 2010·European Journal of Medical Research·Elzbieta GorskaM Wasik
May 28, 2020·International Journal of Molecular Sciences·Michal DulibanBarbara Bilinska
Feb 10, 1999·The American Journal of Physiology·J M BrysonG J Cooney
Jan 14, 1999·The American Journal of Physiology·C M AshwellJ P McMurtry
Jun 10, 2000·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·H F WildmanG P Smith
Nov 14, 2019·International Journal of Molecular Sciences·Thomas Ho-Yin LeeSuk-Yu Yau
Jan 12, 1999·Protein Expression and Purification·N RaverA Gertler

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