Leptin Modulates Mitochondrial Function, Dynamics and Biogenesis in MCF-7 Cells

Journal of Cellular Biochemistry
M Mar Blanquer-RossellóAdamo Valle

Abstract

The adipokine leptin, known for its key role in the control of energy metabolism, has been shown to be involved in both normal and tumoral mammary growth. One of the hallmarks of cancer is an alteration of tumor metabolism since cancerous cells must rewire metabolism to satisfy the demands of growth and proliferation. Considering the sensibility of breast cancer cells to leptin, the objective of this study was to explore the effects of this adipokine on their metabolism. To this aim, we treated the MCF-7 breast cancer cell line with 50 ng/mL leptin and analyzed several features related to cellular and mitochondrial metabolism. As a result, leptin increased cell proliferation, shifted ATP production from glycolysis to mitochondria and decreased the levels of the glycolytic end-product lactate. We observed an improvement in ADP-dependent oxygen consumption and an amelioration of oxidative stress without changes in total mitochondrial mass or specific oxidative phosphorylation (OXPHOS) complexes. Furthermore, RT-PCR and western blot showed an up-regulation for genes and proteins related to biogenesis and mitochondrial dynamics. This expression signature, together with an increased mitophagy observed by confocal microscopy suggests...Continue Reading

References

Oct 1, 1993·Analytical Biochemistry·Z M Chrzanowska-LightowlersR N Lightowlers
May 1, 1997·The Journal of Nutrition·W C Willett
Oct 8, 1997·Proceedings of the Society for Experimental Biology and Medicine·M P Cleary, N J Maihle
Aug 29, 1998·British Journal of Haematology·T NakaoN Tatsumi
Oct 6, 1999·European Journal of Obstetrics, Gynecology, and Reproductive Biology·C SchubringW Kiess
Jun 9, 2000·Annual Review of Physiology·R S Ahima, J S Flier
Nov 10, 2001·American Journal of Physiology. Endocrinology and Metabolism·R BergeronG I Shulman
Jan 18, 2002·Nature·Yasuhiko MinokoshiBarbara B Kahn
May 22, 2002·Cellular Signalling·Gary Sweeney
Nov 20, 2002·International Journal of Obesity and Related Metabolic Disorders : Journal of the International Association for the Study of Obesity·S MargeticR A Hill
Nov 21, 2002·Journal of the National Cancer Institute·Xin HuMargot P Cleary
Nov 22, 2002·Proceedings of the National Academy of Sciences of the United States of America·Haihong ZongGerald I Shulman
Feb 24, 1956·Science·O WARBURG
Jan 30, 2004·Recent Progress in Hormone Research·Christian Bjørbaek, Barbara B Kahn
Jul 9, 2004·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Makoto IshikawaHirokazu Nagawa
Aug 24, 2004·Oncogene·Eugenia E Calle, Michael J Thun
Oct 6, 2005·International Journal of Cancer. Journal International Du Cancer·Yasuo MiyoshiShinzaburo Noguchi
Feb 8, 2006·American Journal of Medical Genetics. Part a·M Michael Cohen
Jan 25, 2007·Molecular Cell·Maria Høyer-HansenMarja Jäättelä
May 2, 2007·Proceedings of the National Academy of Sciences of the United States of America·Lindsay M RohasBruce M Spiegelman
Jul 5, 2007·Proceedings of the National Academy of Sciences of the United States of America·Sibylle JägerBruce M Spiegelman
Feb 21, 2008·Diabetes, Obesity & Metabolism· Anubhuti, Sarika Arora
May 20, 2008·Experimental and Toxicologic Pathology : Official Journal of the Gesellschaft Für Toxikologische Pathologie·Marcia Kazumi NagamineMaria Lúcia Zaidan Dagli
Jan 14, 2009·Seminars in Cancer Biology·Stéphanie GrandemangeJean-Claude Martinou
Jul 23, 2009·Proceedings of the National Academy of Sciences of the United States of America·Amandeep SinghJeffrey Friedman
Nov 9, 2010·The American Journal of Pathology·Jiyoung ParkPhilipp E Scherer
Jan 25, 2011·Nature Cell Biology·Joungmok KimKun-Liang Guan
Jun 15, 2011·Journal of Huazhong University of Science and Technology. Medical Sciences = Hua Zhong Ke Ji Da Xue Xue Bao. Yi Xue Ying De Wen Ban = Huazhong Keji Daxue Xuebao. Yixue Yingdewen Ban·Yi LiuHui Sheng
Jul 6, 2011·The Biochemical Journal·Martin D Brand, David G Nicholls
Aug 31, 2011·Medical Oncology·Mehmet Artac, Kadri Altundag
Nov 15, 2011·Antioxidants & Redox Signaling·Ivana Novak
Feb 1, 2012·Biochimica Et Biophysica Acta·Shanchun GuoRuben R Gonzalez-Perez
Sep 1, 2012·Annual Review of Genetics·David C Chan
Sep 1, 2012·Science·Richard J Youle, Alexander M van der Bliek
Sep 25, 2012·Nature Reviews. Cancer·Douglas C Wallace
Dec 22, 2012·Acta Biochimica Et Biophysica Sinica·Weihua Wu, Shimin Zhao
Jan 3, 2013·Cancer Science·Tomoyoshi Soga
Jun 22, 2013·Autophagy·Jianhui ZhuCharleen T Chu
Sep 5, 2013·Oncology Reports·Jiaode JiangFei Liu

❮ Previous
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Citations

Jun 9, 2015·Journal of Cellular Biochemistry·Vasilij KoshkinSergey N Krylov
Feb 2, 2017·Journal of Neuroinflammation·Fernando BartolomeEva Carro
Oct 6, 2018·Journal of Oral Pathology & Medicine : Official Publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology·Rogério Gonçalves da RochaLucyana Conceição Farias
Jun 13, 2019·Acta Biochimica Et Biophysica Sinica·Qianqian LiuWei Xiong
Oct 1, 2020·Reproductive Sciences·Bruno P MoreiraMarco G Alves
Feb 25, 2017·Current Hypertension Reports·Vicente LaheraLeón Ferder
Nov 9, 2018·International Journal of Molecular Sciences·Monserrat Olea-FloresNapoleón Navarro-Tito
Jan 17, 2020·Scientific Reports·Charlotte CosemansMichelle Plusquin
Apr 19, 2020·Proceedings of the National Academy of Sciences of the United States of America·Mojdeh TavallaieLei Fu
Oct 20, 2017·Oncology Reports·Sinda MahbouliAdrien Rossary
Feb 6, 2020·Journal of the Endocrine Society·Shivraj GrewalRanganath Muniyappa
Feb 5, 2021·International Journal of Molecular Sciences·Duc-Vinh Pham, Pil-Hoon Park
Aug 14, 2021·Journal of Trace Elements in Medicine and Biology : Organ of the Society for Minerals and Trace Elements (GMS)·Somayeh Handali, Mohsen Rezaei

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