Leptin regulates energy metabolism in MCF-7 breast cancer cells

The International Journal of Biochemistry & Cell Biology
Mª Del Mar Blanquer-RossellóPilar Roca

Abstract

Obesity is known to be a poorer prognosis factor for breast cancer in postmenopausal women. Among the diverse endocrine factors associated to obesity, leptin has received special attention since it promotes breast cancer cell growth and invasiveness, processes which force cells to adapt their metabolism to satisfy the increased demands of energy and biosynthetic intermediates. Taking this into account, our aim was to explore the effects of leptin in the metabolism of MCF-7 breast cancer cells. Polarographic analysis revealed that leptin increased oxygen consumption rate and cellular ATP levels were more dependent on mitochondrial oxidative metabolism in leptin-treated cells compared to the more glycolytic control cells. Experiments with selective inhibitors of glycolysis (2-DG), fatty acid oxidation (etomoxir) or aminoacid deprivation showed that ATP levels were more reliant on fatty acid oxidation. In agreement, levels of key proteins involved in lipid catabolism (FAT/CD36, CPT1, PPARα) and phosphorylation of the energy sensor AMPK were increased by leptin. Regarding glucose, cellular uptake was not affected by leptin, but lactate release was deeply repressed. Analysis of pyruvate dehydrogenase (PDH), lactate dehydrogenase (LD...Continue Reading

References

Jan 22, 1992·Biochemical Pharmacology·K LillyL L Bieber
May 1, 1974·The Biochemical Journal·G B Warren, K F Tipton
Oct 8, 1997·Proceedings of the Society for Experimental Biology and Medicine·M P Cleary, N J Maihle
Jun 10, 1998·Biochemical and Biophysical Research Communications·A MartiJ A Martinez
Oct 6, 1999·European Journal of Obstetrics, Gynecology, and Reproductive Biology·C SchubringW Kiess
Jun 9, 2000·Annual Review of Physiology·R S Ahima, J S Flier
Apr 12, 2001·European Journal of Biochemistry·S Rodríguez-EnríquezR Moreno-Sánchez
Jan 18, 2002·Nature·Yasuhiko MinokoshiBarbara B Kahn
Oct 31, 2002·Best Practice & Research. Clinical Rheumatology·Astrid van Tubergen, Alita Hidding
Nov 21, 2002·American Journal of Physiology. Endocrinology and Metabolism·Gregory R SteinbergDavid J Dyck
Mar 12, 2004·The Journal of Surgical Research·Ponnandai SomasundarLinda Vona-Davis
Aug 24, 2004·Oncogene·Eugenia E Calle, Michael J Thun
Jul 21, 2005·International Journal of Obesity : Journal of the International Association for the Study of Obesity·R B Ceddia
Dec 13, 2005·The Biochemical Journal·Gema Frühbeck
Dec 21, 2005·Biochemical and Biophysical Research Communications·Woo Je LeeJoong-Yeol Park
Feb 18, 2006·Annals of Surgical Oncology·Geoffrey A PorterPaul J Veugelers
Feb 17, 2007·The FEBS Journal·Rafael Moreno-SánchezEmma Saavedra
Oct 17, 2007·Breast Cancer Research and Treatment·Bilal MajedBernard Asselain
Feb 8, 2008·Molecular and Cellular Endocrinology·A JanovskáG A Wittert
Feb 21, 2008·Diabetes, Obesity & Metabolism· Anubhuti, Sarika Arora
Apr 4, 2008·Molecular & Cellular Proteomics : MCP·Chun Pong LeeA Harvey Millar
May 20, 2008·Experimental and Toxicologic Pathology : Official Journal of the Gesellschaft Für Toxikologische Pathologie·Marcia Kazumi NagamineMaria Lúcia Zaidan Dagli
Nov 27, 2008·The Journal of Clinical Investigation·Pierre SonveauxMark W Dewhirst
May 23, 2009·Science·Matthew G Vander HeidenCraig B Thompson
May 29, 2010·The Oncologist·Kathleen Y WolinGraham A Colditz
Nov 9, 2010·The American Journal of Pathology·Jiyoung ParkPhilipp E Scherer
Apr 22, 2011·Nature Reviews. Cancer·Willem H KoppenolChi V Dang
Jun 18, 2011·Science·Suman K DasGerald Hoefler
Nov 1, 2011·Nature Medicine·Kristin M NiemanErnst Lengyel
Mar 24, 2012·Cancer Cell·Patrick S Ward, Craig B Thompson
Apr 9, 2013·Indian Journal of Endocrinology and Metabolism·Deep DuttaSubhankar Chowdhury
Oct 12, 2013·Nature Reviews. Drug Discovery·Lorenzo GalluzziGuido Kroemer
Nov 12, 2013·Journal of Mammary Gland Biology and Neoplasia·Neeraj K Saxena, Dipali Sharma
Jul 6, 2014·American Journal of Physiology. Gastrointestinal and Liver Physiology·Huaidong HuHong Ren

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Citations

Jan 25, 2018·Genes·Francesca MastropasquaMaria Shoshan
Dec 1, 2017·Current Pathobiology Reports·Benedicte F JordanPatrice D Cani
Aug 25, 2019·Bioprocess and Biosystems Engineering·Hengfang TangZhiming Zheng
Nov 9, 2018·International Journal of Molecular Sciences·Monserrat Olea-FloresNapoleón Navarro-Tito
Aug 6, 2019·Frontiers in Oncology·Flora Sánchez-JiménezVíctor Sánchez-Margalet
Feb 5, 2021·International Journal of Molecular Sciences·Duc-Vinh Pham, Pil-Hoon Park
May 1, 2021·Nature Reviews. Endocrinology·Kristy A Brown
May 2, 2021·Cancer & Metabolism·Violet A KieselDorothy Teegarden

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