Leptomonas seymouri: Adaptations to the Dixenous Life Cycle Analyzed by Genome Sequencing, Transcriptome Profiling and Co-infection with Leishmania donovani

PLoS Pathogens
Natalya KraevaVyacheslav Yurchenko

Abstract

The co-infection cases involving dixenous Leishmania spp. (mostly of the L. donovani complex) and presumably monoxenous trypanosomatids in immunocompromised mammalian hosts including humans are well documented. The main opportunistic parasite has been identified as Leptomonas seymouri of the sub-family Leishmaniinae. The molecular mechanisms allowing a parasite of insects to withstand elevated temperature and substantially different conditions of vertebrate tissues are not understood. Here we demonstrate that L. seymouri is well adapted for the environment of the warm-blooded host. We sequenced the genome and compared the whole transcriptome profiles of this species cultivated at low and high temperatures (mimicking the vector and the vertebrate host, respectively) and identified genes and pathways differentially expressed under these experimental conditions. Moreover, Leptomonas seymouri was found to persist for several days in two species of Phlebotomus spp. implicated in Leishmania donovani transmission. Despite of all these adaptations, L. seymouri remains a predominantly monoxenous species not capable of infecting vertebrate cells under normal conditions.

References

Nov 1, 1977·The Journal of protozoology·F G Wallace
Aug 1, 1992·Molecular and Biochemical Parasitology·L GuilbrideK Stuart
Jun 1, 1985·Experimental Parasitology·J C Mottram, G H Coombs
Feb 1, 1984·Molecular and Biochemical Parasitology·L J GoadD H Beach
Aug 1, 1980·The Journal of protozoology·M F De SáE S Gander
Apr 1, 1996·The American Journal of Tropical Medicine and Hygiene·G SalinasN Saravia
Jan 29, 2000·The Journal of Eukaryotic Microbiology·J P Dedet, F Pratlong
May 4, 2001·International Journal for Parasitology·S Podlipaev
Jul 16, 2002·Memórias do Instituto Oswaldo Cruz·Marcelo Simão Ferreira, Aércio Sebastião Borges
Apr 16, 2003·FEMS Microbiology Letters·Maria Cristina M MottaMarc Thiry
Sep 4, 2003·Genome Research·Li LiDavid S Roos
Jul 1, 1959·The Journal of Infectious Diseases·R B McGHEE
Aug 1, 1963·Experimental Parasitology·P T JOHNSONM HERTIG
Nov 14, 2003·Kinetoplastid Biology and Disease·Véronique HannaertPaul AM Michels
Jul 27, 2004·Antimicrobial Agents and Chemotherapy·Karen M GrantJeremy C Mottram
Jan 1, 1994·Parasitology Today·G A Schaub
Sep 11, 2004·Current Molecular Medicine·P A Bates, M E Rogers
Jun 9, 2005·Protein Expression and Purification·Susanna KushnirKirill Alexandrov
Jun 28, 2005·Nucleic Acids Research·Peter SchattnerTodd M Lowe
Jul 16, 2005·Science·Najib M El-SayedNeil Hall
Jul 16, 2005·Science·Alasdair C IvensPeter J Myler
Mar 1, 2006·Trends in Parasitology·Alastair G B SimpsonJulius Lukes
Mar 21, 2006·Molecular and Biochemical Parasitology·Fred R Opperdoes, Jean-Pierre Szikora
Jul 18, 2006·Nucleic Acids Research·Mario StankeBurkhard Morgenstern
Feb 27, 2007·Trends in Parasitology·Fred R Opperdoes, Graham H Coombs
Mar 17, 2007·Nature Reviews. Microbiology·Soo Hee LeePaul T Englund
Sep 22, 2007·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Doron RosenzweigDan Zilberstein
Apr 11, 2008·Clinical Microbiology Reviews·Jorge AlvarJavier Moreno
May 1, 2008·Nucleic Acids Research·Stefan GötzAna Conesa
Mar 31, 2009·International Journal of Systematic and Evolutionary Microbiology·Vyacheslav Y YurchenkoDmitri A Maslov
Apr 7, 2009·Transactions of the Royal Society of Tropical Medicine and Hygiene·Narayan Raj BhattaraiJean-Claude Dujardin
Oct 22, 2009·Nucleic Acids Research·Martin AslettHaiming Wang
Dec 9, 2009·International Journal for Parasitology·Ludek KorenýMiroslav Oborník
Feb 2, 2010·The Journal of Eukaryotic Microbiology·Dmitri A MaslovJulius Lukes

❮ Previous
Next ❯

Citations

Aug 6, 2016·Genome Announcements·Lon-Fye LyeStephen M Beverley
Feb 6, 2017·Molecular Aspects of Medicine·Mohammad AkhoundiDenis Sereno
Feb 23, 2018·Proteomics. Clinical Applications·Neeloo SinghEkta Jain
Feb 14, 2018·PloS One·Aygul IshemgulovaVyacheslav Yurchenko
Nov 15, 2016·Evolutionary Bioinformatics Online·Ingrid Thaís Beltrame-BotelhoPatricia Hermes Stoco
Jan 6, 2018·PloS One·Paul Schmid-HempelStefan Zoller
Nov 28, 2018·The Journal of Eukaryotic Microbiology·Jan VotýpkaJulius Lukeš
Oct 12, 2018·Tropical Medicine & International Health : TM & IH·Alexei Yu KostygovVyacheslav Yurchenko
Oct 24, 2015·Parasitology·Angamuthu SelvapandiyanAnuja Krishnan
Dec 30, 2017·Proceedings of the National Academy of Sciences of the United States of America·Danyil GrybchukVyacheslav Yurchenko
Sep 11, 2019·Genome Biology and Evolution·Alejandro Jiménez-GonzálezJan O Andersson
Jan 30, 2020·Pathogens·Lucie PodešvováVyacheslav Yurchenko
Jul 11, 2019·PLoS Neglected Tropical Diseases·Diana Azeredo RangelAndré Luiz Rodrigues Roque
Sep 13, 2019·MSphere·Evgeny GerasimovVyacheslav Yurchenko
Mar 25, 2016·The Journal of Eukaryotic Microbiology·Fred R OpperdoesJulius Lukeš
Oct 18, 2018·MBio·Danyil GrybchukVyacheslav Yurchenko
Dec 13, 2018·Journal of Parasitic Diseases : Official Organ of the Indian Society for Parasitology·Mohsen KalantariKourosh Azizi
Oct 24, 2019·Genes·Yuliya KleschenkoVyacheslav Yurchenko
Jun 10, 2017·Parasites & Vectors·Alexa KauferJoel Barratt
Jul 26, 2017·PLoS Neglected Tropical Diseases·Aygul IshemgulovaVyacheslav Yurchenko
Mar 8, 2018·Memórias do Instituto Oswaldo Cruz·Danyil GrybchukVyacheslav Yurchenko
Oct 12, 2019·BMC Genomics·Anzhelika ButenkoVyacheslav Yurchenko
Feb 7, 2020·Viruses·Danyil GrybchukVyacheslav Yurchenko
Jun 25, 2020·Memórias do Instituto Oswaldo Cruz·Carolina BoucinhaClaudia Masini d'Avila-Levy
Aug 8, 2020·Frontiers in Cellular and Infection Microbiology·Lovlesh ThakurManju Jain
Apr 30, 2020·Pathogens·Ingrid Škodová-SverákováJulius Lukeš
Jan 8, 2021·Parasitology·Petra ČermákováAnton Horváth
Feb 10, 2021·G3 : Genes - Genomes - Genetics·John W DaveyJeremy C Mottram

❮ Previous
Next ❯

Datasets Mentioned

BETA
KP717894
AF153040
KP717896
KP717897
AF047495
KP717898
KP717899
JN848802
EPY16273.1
PRJNA285179

Methods Mentioned

BETA
PCR
electrophoresis
dissection
RNA-seq
fluorescence microscopy
light microscopy

Software Mentioned

BlastP
SE Search Server
TriTrypDB
CLC Genomics Workbench
tRNAscan
Seq
OrthoMCL
Blast2GO
BLAST
BlastX

Related Concepts

Related Feeds

Aminoglycosides (ASM)

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.

Antifungals

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.

Antifungals (ASM)

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.

African Trypanosomiasis

African trypanosomiasis, also known as sleeping sickness, is an insect-borne parasitic disease of humans and other animals. It is caused by protozoa of the species Trypanosoma brucei and almost invariably progresses to death unless treated. Discover the latest research on African trypanosomiasis here.

Aminoglycosides

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.

© 2021 Meta ULC. All rights reserved