PMID: 9638823Jun 25, 1998Paper

Lessons from gene knockouts

Revue Scientifique Et Technique
N Osterrieder, E Wolf

Abstract

The authors describe the technique for the application of homologous recombination in embryonic stem cells, which is now widely used to engineer mice which carry specific knockouts of genes. A summary is given of some of the knowledge of the pathogenesis of and resistance to infections with parasites, bacteria, or viruses which has accumulated during recent years, based on the investigation of knockout mice. Special emphasis is placed on knockout animals which lack components of the cytokine network, lack genes which are critical for the correct presentation of antigens or are deficient in different immune cell subsets. In addition, a brief explanation is offered of the possibilities for inducing targeted deletions or mutations in genes of livestock species (e.g., by nuclear transfer or by mutagenesis using the alkylating agent N-ethyl-N-nitrosourea) which could lead to the breeding of animals which are resistant to infectious diseases in the future.

Citations

Dec 10, 1999·Nature Biotechnology·S E DriverP C Roberts
Dec 14, 2002·Anatomia, histologia, embryologia·Katja PrelleEckhard Wolf
Mar 8, 2002·Human Reproduction and Genetic Ethics·G Badura-Lotter
Mar 26, 2002·Journal of Veterinary Medicine. B, Infectious Diseases and Veterinary Public Health·J RudolphN Osterrieder

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