Let-7-mediated suppression of mucin 1 expression in the mouse uterus during embryo implantation

The Journal of Reproduction and Development
Wilasinee InyawilertPin-Chi Tang

Abstract

Mucin 1 (Muc1) is an integral transmembrane mucin glycoprotein expressed on the apical surface of most epithelia. It is considered to be a barrier to the regulation of embryo implantation by inhibiting attachment of the embryo to the endometrium. Therefore, loss of Muc1 on the surface of uterine epithelial cells is necessary for embryo implantation. Studies have demonstrated that microRNAs (miRNAs) play a key role in enhancing embryo implantation in mammals. In this study, we investigated the regulatory role of two miRNAs (let-7a and let-7b) on the expression of Muc1 in mouse uteri during implantation. Western blotting indicated that Muc1 expression was highest on day1 of pregnancy and constantly decreased thereafter until day 4. In contrast to Muc1 expression, increased expression of let-7a and let-7b was evident on day 4 of pregnancy as measured by real-time reverse transcription-polymerase chain reaction (real-time RT-PCR). We demonstrated direct binding of let-7a and let-7b to the 3'untranslated region of muc1. Furthermore, Muc1 expression was suppressed after transfection of mouse uterine epithelial cells isolated from day 1 of pregnancy with let-7a and let-7b. In summary, the present study provides evidence that Muc1 is a...Continue Reading

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Citations

Dec 29, 2015·American Journal of Reproductive Immunology : AJRI·Weimin LiuWilliam Shu-Biu Yeung
Apr 30, 2017·Molecular Reproduction and Development·Sarah A RobertsonJohn E Schjenken
Jan 1, 2016·Reproduction, Fertility, and Development·Dawit TesfayeKarl Schellander
Nov 23, 2017·Reproductive Biology and Endocrinology : RB&E·Jingjie LiangZhengguang Wang
Jul 25, 2018·International Journal of Molecular Sciences·Alessandro La FerlitaCinzia Di Pietro
Apr 11, 2019·Journal of Cellular Physiology·Mohammad Bakhtiar Hesam ShariatiBehrooz Niknafs
Dec 22, 2017·Beneficial Microbes·G M MoloneyJ F Cryan
Dec 12, 2018·Journal of Cellular Physiology·Naser ShokrzadehBehrooz Niknafs
Feb 7, 2021·Reproduction in Domestic Animals = Zuchthygiene·Yishan SunJingli Sun
Jun 12, 2021·Frontiers in Cell and Developmental Biology·Antonia NavarroAyman Al-Hendy
Aug 28, 2021·Journal of Clinical Medicine·Kamila KolanskaEmile Daraï

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Methods Mentioned

BETA
electrophoresis
scrapings
PCR
transfection

Software Mentioned

ImageJ
SAS

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