Let-7e inhibits TNF-α expression by targeting the methyl transferase EZH2 in DENV2-infected THP-1 cells

Journal of Cellular Physiology
Yingke ZhangJunqi Huang

Abstract

Tumor necrosis factor α (TNFα), an important inflammatory cytokine, is associated with dengue hemorrhagic fever/dengue shock syndrome (DHF/DSS), a severe pathological manifestation of dengue virus (DENV) infection. However, the regulatory mechanism of microRNA on TNFα is currently unknown. Our study showed that the TNFα expression increased immediately and then later decreased, while a marked increase for the miRNA let-7e was detected in dengue virus type 2 (DENV2)-infected peripheral blood mononuclear cells (PBMCs). From this study, we found that let-7e was able to inhibit TNFα expression, but bioinformatics analysis showed that the enhancer of zeste homolog 2 (EZH2) was the potential direct target of let-7e instead of TNFα. EZH2 methyl transferase can produce H3K27me3 and has a negative regulatory role. Using a dual-luciferase reporter assay and Western blotting, we confirmed that EZH2 was a direct target of let-7e and found that siEZH2 could inhibit TNFα expression. In the further study of the regulatory mechanism of EZH2 on TNFα expression, we showed that siEZH2 promoted EZH1 and H3K4me3 expression and inhibited H3K27me3 expression. More importantly, we revealed that siEZH2 down-regulated NF-κB p65 within the nucleus. These...Continue Reading

References

Jan 5, 2002·Journal of Virology·Sang Heui Seo, Robert G Webster
Aug 14, 2003·Human Molecular Genetics·Yoon Jun KimHyoung Doo Shin
Sep 1, 2004·Trends in Biochemical Sciences·Stuart S LevineRobert E Kingston
Jan 11, 2007·The Journal of Biological Chemistry·Yong-Yeon ChoZigang Dong
Feb 20, 2007·Immunity·Konstantin D TaganovDavid Baltimore
Jan 22, 2008·Nature Reviews. Immunology·Harvey F LodishChang-Zheng Chen
May 9, 2008·Current Gastroenterology Reports·Myriam FarahMichael F Byrne
Mar 21, 2009·The Journal of Biological Chemistry·Akina SaitohKazuhisa Nakayama
Aug 25, 2009·Immunity·Ariadne AndroulidakiChristos Tsatsanis
Oct 23, 2009·Current Opinion in Neurobiology·Marion Coolen, Laure Bally-Cuif
Apr 14, 2010·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Cong PengZigang Dong
Jul 20, 2010·Seminars in Cell & Developmental Biology·Michael P East, Richard A Kahn
Jan 18, 2011·The Journal of Biological Chemistry·Zhiqiu ManHye-Won Shin
Mar 4, 2011·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Andrew Chase, Nicholas C P Cross
May 18, 2011·Proceedings of the National Academy of Sciences of the United States of America·Mazhar HussainSassan Asgari
Dec 27, 2011·Molecular Cell·Kambiz MousaviVittorio Sartorelli
Mar 1, 2012·Current Topics in Developmental Biology·Vanessa Mondol, Amy E Pasquinelli
Oct 20, 2012·European Journal of Immunology·Hongbing GuanMitzi Nagarkatti
Oct 23, 2012·Cancer Letters·Wen-Ming Chu
May 3, 2013·PloS One·Ernesto E AmbroggioBruno Goud
May 10, 2013·PLoS Neglected Tropical Diseases·Sing-Sin SamSazaly AbuBakar
Jun 5, 2013·Proceedings of the National Academy of Sciences of the United States of America·Guangmei ZhangSassan Asgari
Aug 7, 2013·The Journal of Immunology : Official Journal of the American Association of Immunologists·Shuai ChenWenlin Huang
Sep 7, 2014·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Wenqiao ZangGuoqiang Zhao
Oct 17, 2014·The Journal of Immunology : Official Journal of the American Association of Immunologists·Daniel Say Liang LimSoon Boon Justin Wong
Dec 17, 2014·The Journal of Infection·Weitao WenMengfeng Li
Jan 16, 2015·International Journal of Medical Sciences·Sing-Sin SamSazaly AbuBakar
Jul 2, 2016·Cell·Anna P Durbin

❮ Previous
Next ❯

Citations

Jan 8, 2020·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Xiaotian DuHongwei Ouyang
Mar 30, 2021·Mediators of Inflammation·Nicholas J Monteleone, Carol S Lutz

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.