let-7i-5p, miR-181a-2-3p and EGF/PI3K/SOX2 axis coordinate to maintain cancer stem cell population in cervical cancer

Scientific Reports
Ravindresh Chhabra

Abstract

The characteristics of cancer stem cells (CSCs) and the genes responsible for their maintenance are highly variable in different cancers. Here, we identify the coordination among miRNAs and EGF pathway genes which is critical for the maintenance of CSCs in cervical cancer. The transcript analysis of CSCs enriched from cervical cancer cell lines (CaSki and HeLa) revealed a significant upregulation of SOX2. Since EGF receptor is frequently over expressed in cervical cancer, we hypothesized that EGF pathway may be responsible for the upregulation of SOX2. Also, the media used for CSC enrichment was supplemented with EGF. The hypothesis was validated as inhibiting the EGF/PI3K pathway suppressed the expression of SOX2 and reduced the CSC population. In addition, miRNA profiling identified miR-181a-2-3p and let-7i-5p as markedly reduced in CSCs. The exogenous expression of either of these miRNAs in CaSki cells inhibited the expression of SOX2 and subsequently reduced CSC population. In conclusion, this study highlights for the first time the contrasting role of let-7i-5p/ miR-181a-2-3p and EGF/PI3K/SOX2 axis in maintaining cervical CSCs. While the EGF pathway promotes CSC formation in cervical cancer by inducing SOX2, miR-181a-2-3p/...Continue Reading

References

Nov 2, 2001·Nature·T ReyaI L Weissman
Mar 12, 2003·Proceedings of the National Academy of Sciences of the United States of America·Muhammad Al-HajjMichael F Clarke
Nov 19, 2004·Nature·Sheila K SinghPeter B Dirks
Apr 2, 2005·Nature Reviews. Cancer·Michael DeanSusan Bates
Dec 3, 2005·Cancer Research·Anne T CollinsNorman J Maitland
Nov 24, 2006·Nature·Lucia Ricci-VitianiRuggero De Maria
Jan 19, 2007·The New England Journal of Medicine·Rui LiuMichael F Clarke
Feb 7, 2007·Cancer Research·Chenwei LiDiane M Simeone
Mar 14, 2007·Cancer Cell·Michail ShipitsinKornelia Polyak
Apr 18, 2007·Genes & Development·Yong Sun Lee, Anindya Dutta
Jun 6, 2007·Proceedings of the National Academy of Sciences of the United States of America·Piero DalerbaMichael F Clarke
Jan 4, 2008·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Felix ZeppernickChristel C Herold-Mende
May 31, 2008·Nature Reviews. Cancer·Michael BaumannRichard Hill
Dec 10, 2008·Blood·John E Dick
Sep 10, 2009·Cell·Mark ShackletonSean J Morrison
May 23, 2012·Clinical & Experimental Metastasis·Erhong MengRodney P Rocconi
Jun 27, 2014·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Ravindresh Chhabra, Neeru Saini
Jan 8, 2015·Journal of Cancer Research and Clinical Oncology·Ravindresh Chhabra
Aug 10, 2016·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Qian MeiWeidong Han
Jan 24, 2017·International Journal of Oncology·Xi ZhouZhao Duan
Apr 30, 2017·BMC Cancer·Weiwen WangLong Li

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Citations

Dec 19, 2018·Current Opinion in Oncology·Nabila BerabezMathieu Gabut
Nov 21, 2019·Molecular Medicine Reports·Zhen LiuXiaoxia Hu
Mar 12, 2020·Journal of Cell Communication and Signaling·Yang ZhengXiaomeng Song
Jul 12, 2020·Reproductive Sciences·Cheng-Lei GuYuan-Guang Meng
Dec 1, 2019·Mutagenesis·Alexandra KondelovaPablo Conesa-Zamora
Nov 11, 2019·Molecular Aspects of Medicine·Cornelia BraicuIoana Berindan-Neagoe
Sep 30, 2021·Expert Reviews in Molecular Medicine·Sepideh MirzaeiHaroon Khan

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Methods Mentioned

BETA
Assay
transfection
PCR
electrophoresis

Software Mentioned

R
Bowtie
DESeq

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