PMID: 8589060Jan 1, 1995Paper

Lethal double-stranded RNA processing activity of ribonuclease III in the absence of suhB protein of Escherichia coli

Biochimie
T Inada, Y Nakamura

Abstract

The suhB gene of Escherichia coli has been defined by its mutant allele that suppresses other mutants in secY, rpoH, dnaB, and era. The suhB mutant by itself is cold sensitive, and is shown to have defects in protein synthesis. Starting with the suhB cold-sensitive mutant, cold-resistant suppressors were isolated. These suppressors mapped to the gene rnc encoding RNase III (a double-strand RNA-processing enzyme), and restored normal protein synthesis to the suhB mutants. Two known rnc mutations, rnc70 or rnc105, both defective in RNA cleavage activity, similarly restored growth of suhB. These rnc mutations did not alter the level of suhB expression. These results suggest that wild-type RNase III exerts a lethal effect on E coli upon depletion of SuhB at low temperatures. One explanation is to assume that the double-strand RNA-processing activity of RNase III itself is potentially lethal to E coli and the normal function of SuhB modulates the lethal action of RNase III.

References

Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·F SangerA R Coulson
Aug 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·R A Young, J A Steitz
Nov 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·D St JohnstonM Jantsch
Aug 25, 1990·Nucleic Acids Research·L Krinke, D L Wulff
Dec 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·H NagaiT Yura
Dec 1, 1986·Microbiological Reviews·T C KingD Schlessinger
Apr 20, 1987·Journal of Molecular Biology·W L Downing, P P Dennis
Sep 1, 1989·Journal of Bacteriology·T InadaY Nakamura
May 1, 1989·Journal of Bacteriology·H E TakiffD L Court
Sep 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·S AltuviaA B Oppenheim
Jan 1, 1985·Molecular & General Genetics : MGG·H Nashimoto, H Uchida
Feb 25, 1974·Journal of Molecular Biology·M Blundell, D Kennell
Aug 28, 1971·Journal of Molecular Biology·R L CoffmanA L Koch
Jun 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·G BarryC L Squires
Oct 16, 1980·Biochemical and Biophysical Research Communications·D R Gitelman, D Apirion
Jun 15, 1984·Journal of Molecular Biology·U SchmeissnerD Court
Jan 1, 1983·Molecular & General Genetics : MGG·T Kurihara, Y Nakamura

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Citations

Feb 20, 2010·BMC Microbiology·Farahnaz MovahedzadehNeil G Stoker
Dec 1, 2007·Molecular Microbiology·Jianhua GanXinhua Ji
Sep 29, 2004·FEMS Microbiology Reviews·Ruth DanielsJan Michiels
Mar 16, 2004·Structure·Jaroslaw BlaszczykXinhua Ji
Nov 28, 2015·Current Genetics·Yongxin JinWeihui Wu
Jun 10, 2016·Journal of Cellular Biochemistry·Dongjie FanChuanpeng Liu
Sep 8, 1998·Microbiology and Molecular Biology Reviews : MMBR·M K Berlyn

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