Abstract
Previous animal models of invasive Haemophilus influenzae type b (HITB) infection are characterized by a low mortality rate. We produced a highly lethal infection in CF1 mice using mouse passage, mucin, and hemoglobin to enhance infectivity. Infection by the intraperitoneal route was followed by progressive peritonitis and bacteremia with subsequent HITB infection of the brain and meninges, and death. Death occurred between eight and 72 hours after infection and was associated with 10(6) to 10(9) HITB per ml of blood and with 10(2) to 10(5) HITB per g of brain. Mucin-hemoglobin did not augment HITB growth, but impaired macrophage adherence to glass in vitro, without decreasing cellular viability. In vivo, mucin-hemoglobin decreased the rate of disappearance of 51Cr-labelled HITB from the blood by impairment of hepatic clearance. This technically simple and inexpensive model is useful for the study of HITB infections in which bacterial multiplication, invasion and host lethality are desired features.
References
Dec 1, 1978·The Journal of Pediatrics·R S DaumA L Smith
Apr 1, 1977·The Journal of Infectious Diseases·R H MichaelsR Klaw
Dec 1, 1979·Infection and Immunity·L B CorbeilA I Braude
Feb 1, 1978·Infection and Immunity·G B Hill
Jun 1, 1978·The Journal of Clinical Investigation·S M Payne, R A Finkelstein
Jun 1, 1976·Canadian Journal of Microbiology·G A CalverG Lavergne
Mar 1, 1977·The Journal of Surgical Research·T HauR L Simmons
Oct 11, 1976·Infection and Immunity·F J CrossonE R Moxon
Oct 1, 1974·Antimicrobial Agents and Chemotherapy·R G Dacey, M A Sande
Aug 1, 1973·Infection and Immunity·A L SmithE R Moxon
Feb 1, 1974·The Journal of Infectious Diseases·E R MoxonD H Smith
Aug 1, 1967·The American Journal of the Medical Sciences·D KayeE W Hook
Oct 1, 1971·Infection and Immunity·R Schneerson, J B Robbins
Feb 1, 1968·Archives of Neurology·T B Ducker, R L Simmons
Jan 1, 1965·Acta Pathologica Et Microbiologica Scandinavica·A Lithander
Apr 1, 1967·Journal of Comparative Pathology·G R Smith
Feb 5, 1982·Science·J W EatonJ T Lee
Apr 1, 1982·The Journal of Clinical Investigation·M I MarksA I Braude
Mar 30, 1963·Annals of the New York Academy of Sciences·G H SCHERR
Dec 1, 1964·Journal of General Microbiology·W SIMS
Dec 1, 1951·The Biochemical Journal·H SMITHS PETO
Jun 1, 1948·Bacteriological Reviews·L Olitzki
Oct 13, 1933·Science·C P Miller
Apr 30, 1937·The Journal of Experimental Medicine·L D FothergillC A Chandler
Citations
Jan 1, 1984·Infection·M I Marks
Jan 1, 1988·Transactions of the Royal Society of Tropical Medicine and Hygiene·P R RidelA Capron
Jul 1, 1994·International Journal of Psychophysiology : Official Journal of the International Organization of Psychophysiology·M Molnár
Jan 1, 1994·Diagnostic Microbiology and Infectious Disease·M A CohenS L Yoder
Sep 2, 1999·Infectious Disease Clinics of North America·U Koedel, H W Pfister
Oct 12, 2010·Journal of Global Infectious Diseases·Navid EzraNoah Craft
Feb 1, 1985·Australian and New Zealand Journal of Medicine·W W Koo
May 8, 2013·Biotechnic & Histochemistry : Official Publication of the Biological Stain Commission·D UluisikT Ozaydın
Jan 9, 2018·PloS One·Emily J OhneckLuis A Actis