Lethal P-lacZ insertion lines expressed during pattern respecification in the imaginal discs of Drosophila

Genome Génome / Conseil National De Recherches Canada
M A RussellS Scanga

Abstract

The imaginal discs of Drosophila are a useful experimental system in which we can study the origin and genetic determination of spatial patterns in development. This involves the separation of the disc-cell population into distinct lineage compartments, based on clonally transmitted expression states of a number of known selector genes. However, these commitments can be abrogated and the compartment boundaries redeployed, when repatterning occurs in cultured disc fragments. This has so far only been explained using the idea of positional information. The genetic basis of this property of the imaginal disc system and its relationship to compartments have not been identified. Here we have screened over 470 recessive lethal P-lacZ enhancer-trap insertions from the Berkeley Drosophila Genome Project for expression after cell death, which initiates pattern respecification in the imaginal discs. The positive lines obtained identify essential genes that may be important for pattern formation. Most show patterned imaginal disc expression, and many have maternal or zygotic effects on embryonic development. One is an allele of schnurri, a gene that encodes a component of the decapentaplegic (dpp) signal transduction pathway used for posi...Continue Reading

References

Jun 1, 1976·Developmental Biology·P A Lawrence, G Morata
Feb 1, 1989·Genes & Development·C Karmazyn-CampelliP Stragier
Apr 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·A Shearn, A Garen
Apr 1, 1995·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·S S Blair
Mar 1, 1995·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·K VijayRaghavan
Jun 1, 1997·Trends in Genetics : TIG·K McCall, H Steller

❮ Previous
Next ❯

Citations

Feb 13, 1999·American Journal of Physical Anthropology·E Eller
Nov 16, 2010·Genome Génome / Conseil National De Recherches Canada·Chris DuranDavid Edwards
Sep 1, 2012·Annual Review of Genetics·Melanie I WorleyIswar K Hariharan
Feb 4, 2010·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Cora BergantiñosFlorenci Serras
Nov 27, 2007·Mechanisms of Development·Kimberly D McClure, Gerold Schubiger
Nov 27, 2007·Mechanisms of Development·Chieka MinakuchiLynn M Riddiford
Aug 22, 2006·Current Biology : CB·Brent S WellsLaura A Johnston
Oct 4, 2002·Developmental Cell·Matthew C GibsonGerold Schubiger
Mar 11, 2010·Development·Cora BergantiñosFlorenci Serras

❮ Previous
Next ❯

Related Concepts

Related Feeds

CREs: Gene & Cell Therapy

Gene and cell therapy advances have shown promising outcomes for several diseases. The role of cis-regulatory elements (CREs) is crucial in the design of gene therapy vectors. Here is the latest research on CREs in gene and cell therapy.