Lethality of Rw/Rw mouse embryos during early postimplantation development

Developmental Biology
M BućanC W Lo

Abstract

Three mutations in the mouse, white spotting (W), rump white (Rw), and patch (Ph), are described as a "gene triplet" on the basis of their close genetic linkage and similar mutant phenotypes. The finding that the W phenotype results from mutations altering the c-kit protooncogene, and that Ph is associated with the deletion of Pdgfra, suggested specific molecular reagents which could be used for the analysis of the chromosomal structure of the third mutation, rump white. Such studies indicated that Rw is associated with a large chromosomal inversion. In this study, we showed that it is possible to generate molecular markers specific for the Rw chromosome, as recombination is suppressed between the inverted portion of the Rw chromosome and the wild-type homologue. Using one such marker, we were able to genotype the offspring of Rw/+ intercrosses. This enabled us to show that Rw homozygote embryos die around 9.5 days of gestation. Histological analysis revealed that the embryos undergo gastrulation, forming three germ layers, and in some cases, exhibit a defined axial midline with an apparent notochordal plate. However, mutant embryos are significantly smaller than the wild-type, with the size difference evident from Day 7.5 and ...Continue Reading

Citations

Jul 15, 1998·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·D C BennettA Bernstein
Nov 13, 1998·Proceedings of the National Academy of Sciences of the United States of America·R B HoughM Bućan
Feb 1, 1997·Alimentary Pharmacology & Therapeutics·S A Mitchell, R W Chapman

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