Letrozole stimulates the growth of human endometrial explants cultured in three-dimensional fibrin matrix

Fertility and Sterility
Mozafar KhazaeiRobert F Casper

Abstract

To investigate the effects of an aromatase inhibitor, letrozole, on the growth of human endometrium in a three-dimensional fibrin matrix model of endometriosis. Experimental study of human endometrial biopsies in a three-dimensional fibrin matrix culture system. Academic research center. Eight normal women with benign gynecologic problems. Endometrial biopsy samples were washed, cut into small pieces, and placed between two layers of fibrin gel in the presence or absence of letrozole in the culture medium. Tissue changes were assessed by histological and immunohistochemical staining using an inverted microscope, image analysis, and a semiquantitative scoring system. Stromal and epithelial cell outgrowth into the fibrin matrix and angiogenesis comprising endothelial cell invasion of the matrix. Letrozole (0.1 micromol/L, 1 micromol/L, and 10 micromol/L) exerted a significant growth stimulation effect on endometrial tissue in this model. In contrast to our expectations, letrozole stimulated growth of normal human endometrium in an in vitro model of endometriosis. Normal endometrium may respond differently than endometriotic lesions to therapeutic agents. Our findings should be kept in mind when considering future research to expl...Continue Reading

References

Jan 16, 1993·BMJ : British Medical Journal·M P VesseyR Painter
Dec 22, 1999·Human Reproduction·J T AwwadK Isaacson
Apr 13, 2002·Annals of the New York Academy of Sciences·Asgerally T FazleabasSerdar Bulun
Jun 14, 2002·Journal of Reproductive Immunology·Serdar E BulunSijun Yang
Jul 19, 2005·The Journal of Steroid Biochemistry and Molecular Biology·Serdar E BulunZhihong Lin
May 2, 2006·Fertility and Sterility·Erkut Attar, Serdar E Bulun
Sep 23, 2006·Journal of the Society for Gynecologic Investigation·Murat UlukusAydin Arici
Nov 14, 2006·Fertility and Sterility·Navid EsfandiariRobert F Casper
Dec 13, 2006·American Journal of Reproductive Immunology : AJRI·Navid EsfandiariRobert F Casper
Feb 20, 2007·Annals of the New York Academy of Sciences·Dimitrios MilingosMichael Koutsilieris

❮ Previous
Next ❯

Citations

Jun 1, 2010·Radiation Oncology·A Gabriella WernickeLawrence Kenyon
May 10, 2012·PloS One·Masanori OnoSerdar E Bulun
May 25, 2012·PloS One·Angélique GuillaudeauFrançois Labrousse
Dec 4, 2012·Fertility and Sterility·Israel OrtegaAntoni J Duleba
Sep 4, 2012·Fertility and Sterility·Sérgio Reis SoaresAntonio Pellicer
Aug 1, 2010·Hormone Molecular Biology and Clinical Investigation·Jorge R Pasqualini, Gérard S Chetrite

❮ Previous
Next ❯

Related Concepts

Related Feeds

Blood Clotting Disorders

Thrombophilia includes conditions with increased tendency for excessive blood clotting. Blood clotting occurs when the body has insufficient amounts of specialized proteins that make blood clot and stop bleeding. Here is the latest research on blood clotting disorders.