LETTER TO THE EDITOR Performance of the ViroSeq® HIV-1 Genotyping System v2.0 in Central Africa

The Open AIDS Journal
Linda Chapdeleine Mekue MouafoLaurent Bélec

Abstract

Resistance genotypes in pol gene of HIV-1 were obtained by the ViroSeq(®) HIV-1 Genotyping System v2.0 (Celera Diagnostics, Alameda, CA, USA) in 138 of 145 (95%) antiretroviral treatment-experienced adults in virological failure living in Central Africa (Cameroon, Central African Republic, Chad, Gabon). HIV-1 group M exhibited broad genetic diversity. Performance of the 7 ViroSeq(®) sequencing primers showed high failure rate, from 3% to 76% (D: 76%; F: 17%; A and H: 15%; G and B: 4%; C: 3%). These findings emphasize the need of updating the ViroSeq(®) HIV-1 genotyping system for non-B subtypes HIV-1.

References

Jul 5, 2003·Journal of Clinical Microbiology·Diana D HuangJames W Bremer
Apr 28, 2004·Journal of Virological Methods·Bart MaesKristel Van Laethem
Jul 24, 2010·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·M PeetersE Delaporte
May 23, 2012·PloS One·Serge C BillongJean B Elat Nfetam
Dec 26, 2012·Journal of Virological Methods·Moussa ThiamCoumba Toure-Kane

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Citations

Feb 24, 2016·Nigerian Medical Journal : Journal of the Nigeria Medical Association·Mohammad-Ali JenabianLaurent Bélec
Jan 7, 2017·Current Opinion in HIV and AIDS·Urvi M ParikhJohn W Mellors
Feb 25, 2019·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·Junpeng ZhaoLunan Wang

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Datasets Mentioned

BETA
KF735813
JF803976
FJ688173
KC978895
KC978921
KC991139
KF889013
KF889049

Methods Mentioned

BETA
genotyping
PCR

Software Mentioned

ViroSeq ® HIV - 1 Genotyping System
ViroSeq
REGA HIV - 1 & 2 Automated Subtyping Tool

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