Leucine auxotrophy restricts growth of Mycobacterium bovis BCG in macrophages.

Infection and Immunity
F C BangeW R Jacobs

Abstract

The ability of slow-growing mycobacteria to replicate within host mononuclear phagocytes is thought to be central to the pathogenesis of mycobacterial infection. However, because of the lack of a mycobacterial mutant defective for intracellular replication, it has not been possible to test this hypothesis directly. Previously, we showed that a BCG leucine auxotroph with a transposon disruption of the leuD gene is unable to grow in mice. Here we demonstrate that this mutant is also incapable of replicating within cultured macrophages in vitro. Complementation of the leuD mutation with the leuCD genes of Escherichia coli restored wild-type levels of growth in macrophages, establishing that the defect for intracellular replication was due to leucine auxotrophy per se and not to a polar effect of the transposon insertion on an adjacent gene. These results suggest that the inability of the leucine auxotroph to grow in mice was due to its sequestration, after phagocytosis, in an intracellular compartment from which it could not obtain leucine.

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Citations

Dec 24, 2005·Expert Review of Vaccines·Jayne C Hope, H Martin Vordermeier
Jun 21, 2014·BioMed Research International·A Cecilia Helguera-RepettoJorge A Gonzalez-y-Merchand
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May 1, 2015·Clinical and Vaccine Immunology : CVI·Bryan E HartRichard Frothingham
Feb 9, 2017·Memórias do Instituto Oswaldo Cruz·Caroline RizziOdir Antônio Dellagostin
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Sep 10, 2019·The Journal of Infectious Diseases·Yinjuan SongXiangmei Zhou
Apr 24, 2021·The Journal of Infection·Yinjuan SongXiangmei Zhou

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