Leucine-enriched essential amino acids attenuate muscle soreness and improve muscle protein synthesis after eccentric contractions in rats

Amino Acids
Hiroyuki KatoHisamine Kobayashi

Abstract

Eccentric exercise results in prolonged muscle weakness and muscle soreness, which are typical symptoms of muscle damage. Recovery from muscle damage is related to mammalian target of rapamycin (mTOR) activity. Leucine-enriched essential amino acids (LEAAs) stimulate muscle protein synthesis via activation of the mTOR pathway. Therefore, we investigated the effect of LEAAs on muscle protein synthesis and muscle soreness after eccentric contractions (EC). Male Sprague-Dawley rats (9-11 weeks old) were administered an LEAA solution (AminoL40; containing 40 % leucine and 60 % other essential amino acids) at 1 g/kg body weight or distilled water (control) 30 min before and 10 min after EC. Tibialis anterior (TA) muscle was exposed to 500 EC by electrical stimulation under anesthesia. The fractional synthesis rate (FSR; %/h) in the TA muscle was measured by incorporating L-[ring-(2)H5] phenylalanine into skeletal muscle protein. Muscle soreness was evaluated by the paw withdrawal threshold using the Randal-Selitto test with some modifications from 1 to 3 days after EC. The FSR in the EC-control group (0.147 ± 0.016 %/h) was significantly lower than in the sedentary group (0.188 ± 0.016 %/h, p < 0.05). AminoL40 administration signifi...Continue Reading

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Citations

Apr 26, 2016·BioMed Research International·Eleonora BaldissarroSimona Viglio
Jul 22, 2017·Journal of Applied Physiology·Riki OgasawaraNaokata Ishii
Apr 3, 2019·International journal for vitamin and nutrition research. Internationale Zeitschrift für Vitamin- und Ernährungsforschung. Journal international de vitaminologie et de nutrition·Michael V FedewaCollin A Fuqua
Jun 8, 2019·International Journal of Sports Physiology and Performance·Adam D OsmondEdward Jo
Apr 19, 2018·Aging Clinical and Experimental Research·Roberto AquilaniFederica Boschi
Feb 19, 2019·Journal of Physical Therapy Science·Yasushi MatsuiShumpei Miyakawa

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Methods Mentioned

BETA
protein assay
electrophoresis
ELISA

Software Mentioned

GraphPad
GraphPad Prism

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