Leucine metabolism in TNF-alpha- and endotoxin-treated rats: contribution of hepatic tissue

The American Journal of Physiology
M HolecekM Pecka

Abstract

The effects of tumor necrosis factor-alpha (TNF-alpha; cachectin) and lipopolysaccharide of Salmonella enteritidis (LPS; endotoxin) on leucine metabolism in rats were evaluated in the whole body using intravenous infusion of L-[1-14C]leucine and in isolated perfused liver (IPL) using the single-pass perfusion technique with alpha-keto[1-14C]isocaproate as a tracer for measurement of ketoisocaproic acid (KIC) oxidation, and the recirculation technique for measurement of hepatic amino acid exchanges. The data obtained in TNF-alpha and LPS groups were compared with those obtained in controls. Both TNF-alpha and LPS treatment induced an increase of whole body leucine turnover, oxidation, and clearance. As the result of a higher increase of leucine oxidation than of incorporation into the pool of body proteins, the fractional oxidation of leucine was increased. The fractional rate of protein synthesis increased significantly in the spleen (both in TNF-alpha and LPS rats), in blood plasma, liver, colon, kidneys, gastrocnemius muscle (in LPS rats), and in lungs (TNF-alpha-treated rats), whereas it decreased in the jejunum (LPS rats). In IPL of TNF-alpha- and LPS-treated rats a decrease of KIC oxidation and higher uptake of branched-ch...Continue Reading

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Citations

Nov 30, 2018·Annual Review of Physiology·Michael NeinastZoltan Arany
Aug 20, 2002·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Robert A FrostCharles H Lang
Jan 15, 2002·American Journal of Physiology. Endocrinology and Metabolism·Charles H LangThomas C Vary
Nov 27, 2015·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·Luciana RosaEmilio L Streck
Aug 27, 2021·Metabolites·Jüri LiebergJaak Kals

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