Leucine modulates contraction- and insulin-stimulated glucose transport and upstream signaling events in rat skeletal muscle

Journal of Applied Physiology
Nobumasa IwanakaTatsuya Hayashi

Abstract

Leucine has profound effects on glucose metabolism in muscle; however, the effects of leucine on glucose transport in muscle have not been well documented. We investigated the effects of leucine on contraction- and insulin-stimulated glucose transport in isolated rat epitrochlearis muscle in vitro. In the absence of insulin, tetanic contraction increased 3-O-methyl-D-glucose (3-MG) transport and Thr(172) phosphorylation of the catalytic alpha-subunit of 5'-AMP-activated protein kinase (AMPK), a signaling intermediary leading to insulin-independent glucose transport. Leucine (2 mM, 30 min) significantly enhanced contraction-stimulated 3-MG transport and AMPK phosphorylation, accompanied by increased phosphorylation of p70 S6 kinase (p70S6K) Thr(389). The stimulatory effects of leucine on 3-MG transport and AMPK phosphorylation were canceled by STO-609 blockade of Ca(2+)/calmodulin-dependent protein kinase kinase (CaMKK) or rapamycin blockade of p70S6K. On the other hand, leucine blunted insulin-stimulated 3-MG transport and reduced insulin-stimulated Akt Thr(473) phosphorylation. Leucine increased insulin-stimulated p70S6K Thr(389) phosphorylation and enhanced the inhibitory phosphorylation of the insulin receptor substrate 1 (I...Continue Reading

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Citations

Nov 24, 2011·Journal of the International Society of Sports Nutrition·Tsong-Rong JangChen-Kang Chang
Jul 21, 2011·Amino Acids·Fengna LiGuoyao Wu
Jun 5, 2013·Cell Biochemistry and Biophysics·Chiranjib ChakrabortyS A Syed Haneef
Oct 20, 2015·Amino Acids·Nicholas P Gannon, Roger A Vaughan
Apr 9, 2015·Frontiers in Physiology·Carolina B RibeiroCarlos A da Silva
Nov 2, 2017·Applied Physiology, Nutrition, and Metabolism = Physiologie Appliquée, Nutrition Et Métabolisme·Yutaka MatsunagaHideo Hatta
Jan 30, 2018·Molecular Nutrition & Food Research·Nicholas P GannonRoger A Vaughan
May 31, 2018·Applied Physiology, Nutrition, and Metabolism = Physiologie Appliquée, Nutrition Et Métabolisme·Tyler BarnesMarina Mourtzakis
Apr 30, 2011·Journal of Applied Physiology·Maximilian KleinertJohn L Ivy
Jan 7, 2014·Medicine and Science in Sports and Exercise·Jochen KresslerMark S Nash
Aug 5, 2016·American Journal of Physiology. Cell Physiology·Mahshid MogheiOlasunkanmi A J Adegoke
Aug 7, 2020·Applied Physiology, Nutrition, and Metabolism = Physiologie Appliquée, Nutrition Et Métabolisme·Oana AncuRichard W Mackenzie

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