PMID: 4894692Apr 1, 1969Paper

Leucine tRNA and cessation of Escherichia coli protein synthesis upon phage T2 infection

Proceedings of the National Academy of Sciences of the United States of America
T Kano-Sueoka, N Sueoka

Abstract

The involvement of tRNA in cellular differentiation has been tested by analyzing aminoacyl-tRNA of Escherichia coli after phage T2 infection. One or two minutes after infection, half of one of the five leucine tRNA components (Leu-tRNA(1), CUG responding) undergoes a drastic structural change which leads to inactivity of both leucine acceptor activity and codon response. Whether or not the modification causes cessation of host protein synthesis without inhibiting phage-specific protein synthesis has been examined by analyzing polysome-bound leucine tRNA of E. coli before and after the phage infection. The results presented in this paper indicate that the amount of Leu-tRNA(1) used after infection was greatly reduced as compared to that used in noninfected cells. Studies of the in vitro protein-synthesizing system show that T2 mRNA rarely contains the CUG codon. A mechanism by which host mRNA translation is inhibited by the phage infection is proposed from this available information.

References

Jan 1, 1966·Cold Spring Harbor Symposia on Quantitative Biology·F O Wettstein
Aug 14, 1968·Journal of Molecular Biology·S Hattman, P H Hofschneider
Sep 1, 1968·Proceedings of the National Academy of Sciences of the United States of America·S B WeissN H Scherberg
Nov 14, 1968·Journal of Molecular Biology·T Kano-Sueoka, N Sueoka
Jul 14, 1968·Journal of Molecular Biology·T Kano-SueokaN Sueoka
Sep 1, 1966·Journal of Molecular Biology·T Kano-Sueoka, N Sueoka
Oct 1, 1963·Journal of Molecular Biology·M CANNONW GILBERT
Dec 1, 1964·Proceedings of the National Academy of Sciences of the United States of America·N SUEOKA, T KANO-SUEOKA

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Citations

Sep 1, 1973·Virology·G C Schito
Apr 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·W M HolmesG W Hatfield
Jun 3, 2015·Molecular Microbiology·Lital BittonGabriel Kaufmann
Sep 1, 1974·Journal of Bacteriology·M J TevethiaM Mandel
Jun 1, 1979·Microbiological Reviews·J F Koerner, D P Snustad

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