Leucine zippers of fos, jun and GCN4 dictate dimerization specificity and thereby control DNA binding

Nature
T Kouzarides, E B Ziff

Abstract

The products of the fos and jun protooncogenes form a stable heterodimer which binds to the TPA-responsive element (TRE) TGACTCA with high affinity. These two proteins, together with the yeast GCN4 protein, belong to a growing family of transcription factors, including FosB, Fra1, JunB and JunD, whose members share a highly conserved DNA-binding domain. This domain is composed of two structures: a basic motif, which is thought to bind directly to DNA; and a leucine zipper, which provides a dimerization interface. Although this domain is highly conserved in Fos, Jun and GCN4, each of these three proteins has very different relative affinities for the TRE. To understand these differences, we used 'domain-swapping' experiments designed to test the relative contributions of the basic motif and the leucine zipper to TRE-binding affinity. Here we show that fos, jun and GCN4 have different affinities for the TRE due to differences in the hetero- or homo-dimerization capacity of their leucine zipper domains; the basic motifs of these three proteins have comparable DNA binding potential. These results indicate that leucine zippers control the types of protein complexes which can associate with a TRE and regulate gene expression.

References

Dec 1, 1988·Genes & Development·F J RauscherT Curran
Mar 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·K RyderD Nathans
Mar 1, 1989·Journal of Cellular Physiology·R J VosatkaE B Ziff
Jan 27, 1989·Science·E K O'SheaP S Kim
Dec 15, 1988·Nature·T Kouzarides, E Ziff
May 1, 1988·Molecular and Cellular Biology·D R Cohen, T Curran
Mar 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·K RyderD Nathans
May 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·P K VogtR F Doolittle
Jan 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·T A Kunkel

❮ Previous
Next ❯

Citations

Sep 20, 1995·Molecular & General Genetics : MGG·T KusanoK Sugawara
Dec 1, 1992·The International Journal of Biochemistry·W M Krajewska
Sep 23, 1993·Biochimica Et Biophysica Acta·K A Lee, N Masson
May 1, 1992·Immunology Today·N S Greenspan, L J Cooper
Sep 1, 1991·Brain Research. Molecular Brain Research·M SimonatoJ O McNamara
Dec 10, 1991·Biochimica Et Biophysica Acta·P Angel, M Karin
Mar 5, 2013·The International Journal of Biochemistry & Cell Biology·Weishan HuangAvery August
Apr 28, 2001·Molecular and Cellular Endocrinology·D BecquetA M François-Bellan
Aug 22, 2002·Nature Reviews. Cancer·Peter K Vogt
May 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·L J RansoneI M Verma
Aug 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·M L ShermanD W Kufe
May 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·E KerkhoffK H Klempnauer
Aug 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·W T Pu, K Struhl
Sep 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·T Deng, M Karin
Mar 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·D TzamariasK Struhl
Oct 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·M Garcia, J Samarut
Mar 29, 1994·Proceedings of the National Academy of Sciences of the United States of America·A E Menkens, A R Cashmore
Jul 9, 2009·The Journal of Biological Chemistry·Aslihan OrsHiroyuki Yamano
Sep 12, 2008·The Journal of Biological Chemistry·Pilar M CrespoBeatriz L Caputto
Jan 1, 1996·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·B LiuR L Whisler
Jul 25, 1992·Nucleic Acids Research·W J van HeeckerenK Struhl
Sep 11, 1993·Nucleic Acids Research·W T Pu, K Struhl
May 1, 1992·Molecular Microbiology·E A Raleigh
Jan 1, 1990·Acta Paediatrica Scandinavica. Supplement·N L Eberhardt
Oct 13, 1992·Annals of the New York Academy of Sciences·M U NollertL V McIntire

❮ Previous
Next ❯

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