Leucocyte activation by anti-lactoferrin antibodies bound to vascular endothelium

Clinical and Experimental Immunology
E PeenT Skogh

Abstract

Human polymorphonuclear neutrophil leucocytes (PMNL) prestimulated with the formylated tripeptide f-Met-Leu-Phe (fMLP) were activated to an immediate chemiluminescence (CL) response by polyclonal rabbit antibodies against human lactoferrin (Lf). This activation, indicating the formation of reactive oxygen species, was induced by intact IgG antibodies but could not be brought about by F(ab')2 fragments. Human Lf was also shown to adhere to the surface of cultured bovine aorta endothelial cells (BEC). When Lf-coated BEC grown on microcarrier beads were reacted with anti-Lf antibodies, an immediate CL response was achieved also with nonprimed PMNL. Here, too, the reaction required intact IgG antibodies. Also, patient sera containing anti-Lf autoantibodies of IgG class were shown to activate fMLP-treated PMNL. The same effect was obtained (in a dose-dependent manner) with the gammaglobulin fraction from anti-Lf-positive serum. Further, anti-Lf-antibody-positive patient sera incubated with Lf-coated BEC beads were also able to activate non-stimulated PMNL to a chemiluminescence response. The results are discussed in relation to possible mechanisms of cell/tissue damage induced by anti-neutrophil cytoplasmic antibodies (ANCA).

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Citations

Aug 8, 1998·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·B TerjungU Spengler
May 26, 1999·Clinical and Experimental Immunology·C Roozendaal, C G Kallenberg
Jan 12, 2000·American Journal of Veterinary Research·S D SwainM T Quinn
Aug 9, 2001·Best Practice & Research. Clinical Gastroenterology·B Terjung, H J Worman
Feb 16, 2002·Clinical Immunology : the Official Journal of the Clinical Immunology Society·J BartůnkováE Hölzelová
May 4, 1999·Clinical and Diagnostic Laboratory Immunology·B Zweiman, C von Allmen
Jan 1, 2004·Human Immunology·Dominique ReumauxDirk Roos

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