Leucocyte and Platelet-rich Fibrin: a carrier of autologous multipotent cells for regenerative medicine

Journal of Cellular and Molecular Medicine
Rosa Di LiddoPier Paolo Parnigotto

Abstract

The wound healing is a complex process wherein inflammation, proliferation and regeneration evolve according to a spatio-temporal pattern from the activation of coagulation cascade to the formation of a plug clot including fibrin matrix, blood-borne cells and cytokines/growth factors. Creating environments conducive to tissue repair, the haemoderivatives are commonly proposed for the treatment of hard-to-heal wounds. Here, we explored in vitro the intrinsic regenerative potentialities of a leucocyte- and platelet-rich fibrin product, known as CPL-MB, defining the stemness grade of cells sprouting from the haemoderivative. Using highly concentrated serum-based medium to simulate wound conditions, we isolated fibroblast-like cells (CPL-CMCs) adhering to plastic and showing stable in vitro propagation, heterogeneous stem cell expression pattern, endothelial adhesive properties and immunomodulatory profile. Due to their blood derivation and expression of CXCR4, CPL-CMCs have been suggested to be immature cells circulating in peripheral blood at quiescent state until activation by both coagulation event and inflammatory stimuli such as stromal-derived factor 1/SDF1. Expressing integrins (CD49f, CD103), vascular adhesion molecules (C...Continue Reading

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Citations

Apr 10, 2019·International Journal of Molecular Sciences·Silvia BarbonRaffaele De Caro
Jan 28, 2020·Journal of Indian Society of Periodontology·Zeinab Rezaei EsfahroodMaryam Shokri
Feb 13, 2019·International Journal of Molecular Sciences·Dorottya KardosIstván Hornyák
Nov 30, 2019·Facial Plastic Surgery Clinics of North America·Aunna PourangKian Karimi
Aug 25, 2020·Tissue & Cell·Ledson Sampaio NogueiraMarcelo Henrique Napimoga
Feb 25, 2021·Tissue Engineering and Regenerative Medicine·Silvia BarbonMaria Teresa Conconi
Apr 13, 2021·Frontiers in Bioengineering and Biotechnology·Xia ZhaoAravind Athiviraham
Aug 6, 2021·Journal of Microscopy and Ultrastructure·Sharmila JasmineMohammad A Alshuniaber
Oct 30, 2021·Tissue Engineering. Part C, Methods·Shimin YuYufeng Zhang

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Methods Mentioned

BETA
Protein Assay
flow cytometry
PCR
Optical microscopy
FACS
Optical

Software Mentioned

ImageLab
FACS Diva

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