Leuconostoc citreum MB1 as biocontrol agent of Listeria monocytogenes in milk

The Journal of Dairy Research
Silvina A PujatoDaniela M Guglielmotti

Abstract

Cell-free supernatant from Leuconostoc citreum MB1 revealed specific antilisterial activity. Preliminary studies demonstrated the proteinaceous, heat-stable, bacteriocin-like trait of the antimicrobial components present in the supernatant. Determination of the genes encoding bacteriocins by PCR and DNA sequencing led to amplification products highly homologous with leucocin A (found in diverse Leuconostoc species) and UviB (found in Leuc. citreum KM20) sequences. Additionally, antimicrobial activity of cell-free supernatant from Leuc. citreum MB1 was revealed by an inhibition halo of the SDS-PAGE gel subjected to a direct detection using Listeria monocytogenes as indicator strain. Different assays were carried out to assess the capacity of Leuc.citreum MB1 to control List. monocytogenes growth: (i) inactivation kinetics of the pathogen by antilisterial compounds present in concentrated cell-free supernatant from Leuc. citreum MB1, (ii) evaluation of optimal Leuc. citreum MB1 initial concentration to obtain maximum List. monocytogenes ATCC 15313 inhibition, and (iii) biocontrol of List. monocytogenes ATCC 15313 with Leuc. citreum MB1 during growth in milk at refrigeration temperature. According to our results, it is unquestiona...Continue Reading

References

Dec 1, 1991·Applied and Environmental Microbiology·H DabaC Lacroix
Oct 1, 1988·Applied and Environmental Microbiology·M J PucciP A Vandenbergh
Sep 1, 1994·Journal of Dairy Science·M E Stiles
Aug 1, 1996·Letters in Applied Microbiology·A M Revol-JunellesG Lefebvre
Jan 21, 2000·FEMS Microbiology Reviews·S EnnaharA Ishizaki
May 2, 2000·Applied and Environmental Microbiology·H L AlakomiI M Helander
Jan 5, 2002·International Journal of Food Microbiology·J ClevelandM L Chikindas
Oct 6, 2005·Nature Reviews. Microbiology·Paul D CotterR Paul Ross
Jun 23, 2007·Journal of Applied Microbiology·G CataldoC Longhi
Dec 18, 2007·International Journal of Food Microbiology·L A MellefontT Ross
Feb 19, 2008·Journal of Bacteriology·Jihyun F KimTae Kwang Oh
Jul 16, 2008·International Journal of Food Microbiology·Rosalia TriasLluís Bañeras
Mar 23, 2011·Applied and Environmental Microbiology·Morten KjosDzung B Diep

❮ Previous
Next ❯

Citations

Nov 9, 2018·Frontiers in Microbiology·Karen G JarvisChristopher J Grim
Oct 25, 2020·Applied and Environmental Microbiology·Thao D TranJeffery A McGarvey
Dec 19, 2020·Comprehensive Reviews in Food Science and Food Safety·Mehran MoradiAnita Lotfi

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.