Leukemia inhibitory factor as a mediator of JAK/STAT activation in murine osteoblasts

Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research
C LoweJ W Pike

Abstract

A number of cytokines have been shown to exert their effects via a recently discovered signaling cascade. One step in this pathway is mediated by a family of nonreceptor protein tyrosine kinases, the Janus kinases or JAK kinases, which become phosphorylated upon ligand-receptor binding and receptor phosphorylation. This in turn is followed by phosphorylation of certain members of a family of latent transcription factors, called signal transducers and activators of transcription (STATs), which subsequently enter the nucleus, bind to DNA in a sequence-specific fashion, and modulate transcription. In view of the apparent role of leukemia inhibitory factor (LIF) in bone remodeling, we sought to determine which, if any, of the JAK/STAT family members are involved in mediating the actions of LIF using the MC3T3-E1 cell line (a spontaneously immortalized osteoblast) and normal murine calvarial osteoblasts. We report here rapid and transient phosphorylation of the LIF receptor, and similarly, we detect phosphorylation of predominantly JAK1 and to a minor extent JAK2 in response to LIF treatment in MC3T3-E1 cells. In these experiments we also detect phosphorylation of STAT1 and to a much lesser degree STAT3 upon addition of LIF. Phospho...Continue Reading

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Citations

Oct 3, 2008·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Hui-Wen LoFrancis Ali-Osman
Jun 15, 1997·The Journal of Clinical Investigation·Y FujioT Kishimoto
Feb 7, 2012·Growth Factors·Natalie A Sims, Rachelle W Johnson
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Feb 23, 2020·Journal of Crohn's & Colitis·Alba Garrido-Trigo, Azucena Salas
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Jun 17, 2000·Endocrine Reviews·C J Auernhammer, S Melmed
Oct 12, 2000·Cytokine·D Heymann, A V Rousselle

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