Leukemia inhibitory factor enhances bone formation in calvarial bone defect

The Journal of Craniofacial Surgery
S DazaiT Fujii

Abstract

For bone defect reconstruction, locally administered cytokine plasmid was examined. Leukemia inhibitory factor (LIF) can bind to the osteoblast cell surface and induce bone formation both in vitro and in vivo. The authors investigated the local mouse LIF complementary deoxyribonucleic acid (cDNA) plasmid in the pcDNA 3 expression vector, which is promoted by cytomegalovirus and is stabilized by bovine growth hormone polyadenylation, with a gelatin sponge carrier. A total of 150 male Wistar rats were used. They were divided into three groups. Group 1 (N = 30) was treated with the gelatin carrier of the pcDNA 3 vector, group 2 (N = 90) was treated with three different doses of LIF cDNA (0.1, 1, and 10 micrograms) in the pcDNA 3-vector plasmid along with the gelatin carrier, and group 3 (N = 30) was treated with recombinant human bone morphogenetic protein -2. Ten animals in each group were euthanized at 1, 3, and 5 weeks postoperatively. Animals treated with LIF cDNA showed significantly enhanced bone mineral density (p < 0.05), as confirmed by dual-energy X-ray absorptiometry (DEXA), in 3 weeks compared with the control vehicle. By 3 weeks, the number of fibroblast-like cells and collagen fibers decreased, whereas the osteoblast...Continue Reading

Citations

Feb 11, 2010·Tissue Engineering. Part B, Reviews·Gadi PelledEdward M Schwarz
Feb 7, 2012·Growth Factors·Natalie A Sims, Rachelle W Johnson
Feb 21, 2004·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Nobuhisa KobayashiKyotaro Kanazawa
May 26, 2017·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Masanori KoideNobuyuki Udagawa
Apr 17, 2004·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Sadanori AkitaKozo Akino
Dec 2, 2020·Pharmacology & Therapeutics·Cen ZhangZhaohui Feng

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