Leukemia inhibitory factor (LIF) modulates pro-opiomelanocortin (POMC) gene regulation in stably transfected AtT-20 cells overexpressing LIF

Endocrine
Q L LiS Melmed

Abstract

Leukemia inhibitory factor (LIF) levels are elevated in sepsis and correlate with shock and poor prognosis. We have previously shown that lipopolysaccharide (LPS) administration induces hypothalamic and pituitary LIF expression in vivo, which is associated with the acute rise in circulating adrenocorticotrophic hormone (ACTH) levels. As AtT-20 cells respond to LIF, we established murine LIF (mLIF) stably transfected AtT-20 cell lines to study LIF regulation of pro-opiomelanocortin (POMC) expression and ACTH secretion. Our results show that mLIF transfectants accumulated mLIF (up to 15.6 +/- 3.2 ng/mL after 24 h) as well as increased ACTH secretion (up to 2.4-fold above control cells) in conditioned medium. The magnitude of ACTH induction correlated with mLIF concentrations in different transfectants (r = 0.75-0.88, p < 0.05). Moreover, mLIF transfectants showed a higher sensitivity to CRH stimulation with an increased ACTH production within 8 h (p < 0.05), whereas control cells were responsive to CRH at 24 h. Additionally, mLIF transfectants exhibited a maximum threefold ACTH induction, compared to 1.7-fold in control cells. Furthermore, mLIF transfectants have a blunted dexamethasone-mediated inhibition of ACTH (35% inhibition...Continue Reading

References

Nov 1, 1992·The Journal of Clinical Investigation·P WaringD Metcalf
Sep 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·M M Shen, P Leder
Aug 1, 1991·Endocrine Reviews·R KurzrockM Talpaz
Jun 1, 1995·Current Opinion in Immunology·L M Karnitz, R T Abraham
Aug 16, 1994·Proceedings of the National Academy of Sciences of the United States of America·P H Patterson
Apr 2, 1996·Proceedings of the National Academy of Sciences of the United States of America·E B CullinanC L Stewart
Jun 1, 1996·Cytokine & Growth Factor Reviews·C H Heldin, A Ostman
Oct 29, 1996·Proceedings of the National Academy of Sciences of the United States of America·B StefanaS Melmed

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Citations

Jan 12, 2000·Molecular and Cellular Endocrinology·Q L LiT C Friedman
Feb 25, 2010·Molecular and Cellular Endocrinology·Qin-Yun MaHuai-Dong Song
Jun 17, 2000·Endocrine Reviews·C J Auernhammer, S Melmed
Dec 20, 2000·American Journal of Physiology. Endocrinology and Metabolism·Q L LiT C Friedman

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