Leukocyte activation and flow behavior in rat skeletal muscle in sepsis

American Journal of Respiratory and Critical Care Medicine
R D PiperR F Potter

Abstract

In animal models of endotoxemia, sepsis is associated with the accumulation of leukocytes and altered microvascular perfusion. In order to test the hypothesis that bacterial sepsis upregulates leukocyte-endothelial adhesion, we used intravital microscopy to examine the flow behavior of leukocytes in the postcapillary venules (PCV) of rats made septic by cecal ligation and perforation (CLP). Animals were randomized to CLP or sham study groups and studied 6 h, 24 h, or 48 h later. In postcapillary venules of the extensor digitorum longus muscle, we found that: (1) over the course of the study, leukocyte adhesion and extravasation increased in both experimental groups (analysis of variance [ANOVA], significant time effect: adhesion, p < 0.001; extravasation, p < 0.05); (2) leukocyte adhesion was decreased by CLP treatment (ANOVA, sepsis effect, p = 0.05), particularly after 24 to 48 h of sepsis (ANOVA, sepsis x time interaction, p < 0.05); and (3) the reduction in leukocyte adhesion in CLP animals was associated with a decrease in leukocyte extravasation (ANOVA, sepsis effect, p < 0.01). After correction for the reduction in systemic leukocyte count associated with CLP, the effect of sepsis on leukocyte adhesion and extravasation ...Continue Reading

References

Oct 1, 1992·Journal of Applied Physiology·J R ThomasR K Winn
Jun 1, 1992·Journal of Applied Physiology·J BoczkowskiM Aubier
May 1, 1991·The Journal of Clinical Investigation·M A Perry, D N Granger
Feb 9, 1989·The New England Journal of Medicine·S J Weiss
Aug 28, 1986·The New England Journal of Medicine·J E Rinaldo, R M Rogers
Jul 1, 1995·American Journal of Respiratory and Critical Care Medicine·P C RidingsA A Fowler
Nov 1, 1994·The Journal of Clinical Investigation·C LamW Sibbald
Mar 1, 1994·Journal of Critical Care·R J KorthuisD N Granger
Feb 15, 1996·The Journal of Surgical Research·I FarquharW J Sibbald
Oct 1, 1996·American Journal of Respiratory and Critical Care Medicine·R D PiperR F Potter

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Citations

Aug 31, 2002·Pediatric Pulmonology·Philip J Robinson
Mar 4, 2008·Canadian Journal of Anaesthesia = Journal Canadien D'anesthésie·Christian LehmannDragan Pavlovic
Aug 31, 2000·Critical Care Medicine·P B Anning, T W Evans
Jul 5, 2001·Current Opinion in Critical Care·D De Backer, M J Dubois
Jul 2, 2002·American Journal of Respiratory and Critical Care Medicine·Daniel De BackerJean-Louis Vincent
Aug 30, 2008·American Journal of Respiratory and Critical Care Medicine·Hadj Ahmed MostefaiRamaroson Andriantsitohaina
Sep 17, 2003·Critical Care : the Official Journal of the Critical Care Forum·Ryon M BatemanChristopher G Ellis
Sep 20, 2005·Critical Care : the Official Journal of the Critical Care Forum·Christopher G EllisMichael Sharpe
Nov 15, 2006·Critical Care : the Official Journal of the Critical Care Forum·Christian LehmannJürgen Birnbaum
Nov 24, 2011·Oxidative Medicine and Cellular Longevity·T AngelineA Poornima
May 16, 2015·Annals of Intensive Care·Frédéric PèneUNKNOWN Commission de Recherche Translationnelle de la Société de Réanimation de Langue Française (SRLF)
Sep 9, 2017·International Journal of Molecular Sciences·Ryon M BatemanChristopher G Ellis
Aug 21, 2004·American Journal of Physiology. Heart and Circulatory Physiology·Daniel GoldmanChristopher G Ellis
Apr 15, 2005·Microcirculation : the Official Journal of the Microcirculatory Society, Inc·Vidula VachharajaniD Neil Granger
May 18, 2001·American Journal of Physiology. Heart and Circulatory Physiology·R M BatemanC G Ellis
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