Leukocyte activation and leukocyte procoagulant activities after blood contact with polystyrene and polyethylene glycol-immobilized polystyrene beads

The Journal of Laboratory and Clinical Medicine
M B Gorbet, M V Sefton

Abstract

Beads (45 microm) of polystyrene (PS) and polyethylene glycol modified PS (TentaGel) with an amino or hydroxyl terminal group were incubated with blood to assess the effect of surface area and material chemistry on leukocyte activation. After a 2-hour incubation, blood contact with beads activated leukocytes in the bulk (tissue factor expression, CD11b up-regulation, and association with platelets) independently of material surface chemistry. On the other hand, activation of adherent leukocytes was material dependent. After blood contact with PS, polyethylene glycol-immobilized PS (PS-PEG) and PS-PEG-NH2 beads, CD11b up-regulation in the bulk, platelet-leukocyte aggregates, and leukocyte adhesion were all dependent on surface area, whereas tissue factor (TF) expression was not. Material-induced leukocyte activation in the bulk was also independent of the beads' capacity to activate platelets. However, monocyte adhesion and TF expression on beads appeared to be related to the presence of platelets on the surface. Material-induced TF expression was able to initiate the extrinsic pathway of coagulation, resulting in significant fibrin formation. Although not all of our markers of leukocyte activation varied with material area or c...Continue Reading

Citations

Jan 15, 2008·Langmuir : the ACS Journal of Surfaces and Colloids·Ashwin NairLiping Tang
Apr 14, 2005·Proceedings of the National Academy of Sciences of the United States of America·Benjamin G KeselowskyAndrés J García
Apr 1, 2003·Hemodialysis International·Andrew Davenport
Apr 4, 2007·Hemodialysis International·Karl-Georg Fischer
Jul 27, 2012·Journal of Biomaterials Applications·Xiaojian Chang, Maud Gorbet
Jul 16, 2005·Biomaterials·Maud B Gorbet, Michael V Sefton
Nov 13, 2003·Journal of Biomedical Materials Research. Part a·M B Gorbet, M V Sefton
Sep 10, 2017·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Gemma RadleyCatherine A Thornton
Jul 23, 2003·Journal of Biomedical Materials Research. Part a·Elizabeth A Lester, Julia E Babensee
Jul 20, 2007·Journal of Biomedical Materials Research. Part a·Stacey L Rose, Julia E Babensee
Nov 21, 2007·Journal of Biomedical Materials Research. Part a·Shwetha KamathLiping Tang
Aug 24, 2010·Journal of Biomedical Materials Research. Part a·Manfred F MaitzCarsten Werner
Jan 20, 2005·Journal of Biomedical Materials Research. Part a·Stacey L Rose, Julia E Babensee
Jun 27, 2021·Wiley Interdisciplinary Reviews. Nanomedicine and Nanobiotechnology·Reshma Vasantha RamachandranAmbarish Ghosh
Jul 22, 2006·Biomaterials·Jennifer E PhillipsAndrés J García

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