PMID: 7544340May 1, 1995Paper

Leukocyte adhesion induced by inhibition of nitric oxide production in skeletal muscle

Journal of Applied Physiology
T AkimitsuR J Korthuis

Abstract

Superfusion of rat cremaster muscles with the nitric oxide (NO) synthase inhibitor NG-nitro-L-arginine methyl ester (L-NAME) elicited significant leukocyte adhesion to postcapillary venules (20- to 30-microns diameter), an effect that was attenuated by pretreatment with L-arginine (an NO precursor) or sodium nitroprusside (SNP) (an exogenous source of NO). In contrast to the effects of pretreatment, addition of SNP or L-arginine to the superfusate 30 min after the initiation of NO synthase inhibition failed to reverse the L-NAME-induced leukocyte adherence. However, this effect was reversed by administration of an anti-CD18 monoclonal antibody or 8-bromoguanosine 3',5'-cyclic monophosphate 30 min after L-NAME superfusion was initiated. These findings indicate that L-NAME promotes leukocyte adhesion to venular endothelium by a CD18-dependent mechanism in skeletal muscle and suggest that the failure of L-arginine or SNP to reverse L-NAME-induced leukocyte adherence is not due to a defect in signaling events that occur subsequent to activation of guanylate cyclase by NO derived from these agents. Because the simultaneous administration of superoxide dismutase (scavenges superoxide radicals) and SNP or L-arginine, but not superoxid...Continue Reading

Citations

Jan 12, 2001·Physiological Reviews·J S Stamler, G Meissner
Nov 25, 2000·Laboratory Investigation; a Journal of Technical Methods and Pathology·D HochbergD Felsen
Oct 24, 2002·Clinical Orthopaedics and Related Research·James G Tidball
Aug 5, 1998·The American Journal of Physiology·J G TidballM Wehling
Jan 8, 2005·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·James G Tidball
Dec 15, 2007·The American Journal of Pathology·Sarah NolanVictoria Ridger
Jul 1, 1998·Clinical Immunology and Immunopathology·D S Tews, H H Goebel
Sep 5, 2002·Neuromuscular Disorders : NMD·D S Tews
Dec 8, 2000·Thrombosis Research·S Sethi, M Dikshit
Jan 1, 1997·Kidney International·J RyszE Sibińska

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.