PMID: 9189819Jun 15, 1997Paper

Leukocyte-biomaterial interactions in the presence of Staphylococcus epidermidis: flow cytometric evaluation of leukocyte activation

Journal of Biomedical Materials Research
S SapatnekarJ M Anderson

Abstract

The adhesion of bacteria on a biomaterial surface is believed to be the first step in the development of biomaterial-related infection. The goal of this study was to investigate the mechanisms that permit adherent bacteria to persist on the surface of an implanted cardiovascular biomaterial. We hypothesized that circulating leukocytes are unable to adhere to the biomaterial surface under physiologic shear stress conditions, and this prevents them from interacting with adherent bacteria. To address this hypothesis, we investigated the adhesion profiles of Staphylococcus epidermidis and polymorphonuclear leukocytes (PMN), incubated under controlled shear stress conditions with the test biomaterial. We found that bacteria could adhere on the biomaterial surface, even when their concentration in the test medium was as low as 10(3) cfu/mL. At this concentration, the bacteria did not induce significant complement activation. PMN adhesion on the biomaterial surface was sensitive to shear stress and minimal at shear stress > 10 dynes/cm2. Low concentrations of bacteria could induce a significant increase in the expression of PMN adhesion molecules CD11b and CD11c. We conclude that the presence of bacteria induces PMN activation but doe...Continue Reading

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Citations

Apr 26, 2003·Journal of Biomaterials Science. Polymer Edition·Jasmine D PatelJames M Anderson
Sep 2, 1998·Journal of Biomaterials Science. Polymer Edition·Y ItoY Imanishi
Apr 9, 2002·Journal of Chemotherapy·J M SchierholzG Pulverer
Apr 1, 2005·Journal of Biomedical Materials Research. Part a·Jasmine D PatelJames M Anderson
Jun 16, 2001·Journal of Biomedical Materials Research·O K Muratoglu, W H Harris
Sep 21, 2001·Clinical Oral Implants Research·A A ComutM Spector

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