Leukocyte Expression of Type 1 and Type 2 Purinergic Receptors and Pro-Inflammatory Cytokines during Total Sleep Deprivation and/or Sleep Extension in Healthy Subjects

Frontiers in Neuroscience
M ChennaouiD Gomez-Merino

Abstract

The purinergic type P1 (adenosine A1 and A2A) receptors and the type P2 (X7) receptor have been suggested to mediate physiological effects of adenosine and adenosine triphosphate on sleep. We aimed to determine gene expression of A1R (receptor), A2AR, and P2RX7 in leukocytes of healthy subjects during total sleep deprivation followed by sleep recovery. Expression of the pro-inflammatory cytokines IL-1β and TNF-α were also determined as they have been characterized as sleep regulatory substances, via P2RX7 activation. Blood sampling was performed on 14 young men (aged 31.9 ± 3.9) at baseline (B), after 24 h of sleep deprivation (24 h-SD), and after one night of sleep recovery (R). We compared gene expression levels after six nights of habitual (22.30-07.00) or extended (21.00-07.00) bedtimes. Using quantitative real-time PCR, the amount of mRNA for A1R, A2AR, P2RX7, TNF-α, and IL-1β was analyzed. After 24 h-SD compared to B, whatever prior sleep condition, a significant increase of A2AR expression was observed that returned to basal level after sleep recovery [day main effect, F(2, 26) = 10.8, p < 0.001]. In both sleep condition, a day main effect on P2RX7 mRNA was observed [F(2, 26) = 6.7, p = 0.005] with significant increases ...Continue Reading

References

Jun 1, 1983·Acta Psychiatrica Scandinavica·A S Zigmond, R P Snaith
Sep 25, 2002·Physiological Reviews·R Alan North
Jun 1, 1961·Archives of General Psychiatry·A T BECKJ ERBAUGH
Aug 18, 2004·Progress in Neurobiology·Radhika BasheerRobert W McCarley
Mar 10, 2006·American Journal of Medical Genetics. Part B, Neuropsychiatric Genetics : the Official Publication of the International Society of Psychiatric Genetics·Patrick F SullivanCharles M Perou
Sep 20, 2006·Archives of Internal Medicine·Michael R IrwinSteve Cole
Mar 3, 2007·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·David ElmenhorstAndreas Bauer
Sep 7, 2007·Progress in Neurobiology·Bertil B FredholmMichail Sitkovsky
Nov 17, 2007·Neuroreport·Radhika BasheerRobert W McCarley
Nov 6, 2008·Nature Reviews. Neuroscience·James M KruegerJaak Panksepp
Dec 11, 2008·Proceedings of the National Academy of Sciences of the United States of America·Yo OishiOsamu Hayaishi
Dec 17, 2008·Current Pharmaceutical Design·James M Krueger
Jan 17, 2009·Brain Research·David ElmenhorstAndreas Bauer
Sep 25, 2009·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Katia VaraniPier Andrea Borea
Oct 9, 2009·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Stephen D SkaperPietro Giusti
Sep 11, 2010·Journal of Applied Physiology·James M KruegerMark R Zielinski
Feb 1, 2011·Sleep Medicine Clinics·Christopher J WatsonRalph Lydic
Nov 23, 2012·Journal of Sleep Research·Tracy L RuppThomas J Balkin
May 9, 2013·The European Journal of Neuroscience·James M KruegerDaniel J Buysse
Aug 7, 2013·Brain Research·Youngsoo KimRobert E Strecker
Jan 31, 2014·International Journal of Molecular Sciences·Sandeep ShethVickram Ramkumar
Jun 20, 2014·Neuropsychopharmacology : Official Publication of the American College of Neuropsychopharmacology·Christa HohoffAndreas Bauer
Oct 14, 2014·Metabolism: Clinical and Experimental·Gabriel S Chiu, Gregory G Freund
May 2, 2015·International Journal of Cardiology·Fabien SauvetMounir Chennaoui
May 15, 2015·Frontiers in Neuroscience·Ricardo J RodriguesRodrigo A Cunha
Nov 1, 2015·Neuropharmacology·Ute Krügel
Oct 7, 2016·Nature·Franz Weber, Yang Dan

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Citations

Nov 18, 2017·International Journal of Molecular Sciences·Mounir ChennaouiDanielle Gomez-Merino
Mar 29, 2019·Physiological Reviews·Luciana BesedovskyMonika Haack
Oct 21, 2020·Zhurnal nevrologii i psikhiatrii imeni S.S. Korsakova·M G Poluektov
Jun 29, 2021·Neuroscience and Behavioral Physiology·M G Poluektov

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Methods Mentioned

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PCR
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Software Mentioned

LightCycler
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