Leukocyte immunophenotyping by flow cytometry in a multisite study: standardization, quality control, and normal values in the Transfusion Safety Study. The Transfusion Safety Study Group

Clinical Immunology and Immunopathology
J W ParkerT Odom-Maryon

Abstract

The Transfusion Safety Study (TSS) is a multicenter, cooperative investigation of factors that may determine the occurrence and modify the expression of transfusion-transmitted infections. A flow cytometry laboratory was established in each of the six participating centers in order to avoid alterations in cell phenotypes which may be caused by shipping delays, temperature changes, and handling. As a consequence, in order to assure compatibility of results, stringent standardization, quality control, and proficiency testing procedures were developed. This paper documents (i) the effect of time from phlebotomy to specimen staining and then to analysis for the antibodies used in the study; (ii) the effects of variations in light scatter cursor location for certain antibodies; (iii) a quality control program and data management and analysis system, each specifically designed for the study; and (iv) presents extensive data on age- and sex-related reference (normal) ranges for the several individual and paired monoclonal antibodies used in the study. Problems encountered, including obtaining reliable absolute lymphocyte counts, interference by nucleated erythrocytes, and sources of variability in results, are discussed. This study is...Continue Reading

Associated Clinical Trials

References

Feb 1, 1988·Cellular Immunology·T TakeuchiC Morimoto
Feb 22, 1985·JAMA : the Journal of the American Medical Association·J JasonB Evatt
Nov 1, 1984·The Journal of Clinical Investigation·P L Romain, S F Schlossman
Sep 16, 1983·Journal of Immunological Methods·C F ShieldG Goldstein
Jan 1, 1983·Immunological Communications·J E NagelW H Adler
Sep 29, 1983·The New England Journal of Medicine·B J WeiblenC R Valeri
Jan 1, 1981·The Journal of Experimental Medicine·M KamounR C Nowinski
Apr 1, 1981·Immunology Today·E L Reinherz, S F Schlossman

❮ Previous
Next ❯

Citations

Feb 11, 1993·The New England Journal of Medicine·L M AledortJ W Mosley
Oct 1, 1992·American Journal of Obstetrics and Gynecology·S M BerryJ Kaplan
Apr 24, 2012·Transfusion Medicine·K GutensohnA Humpe
Aug 1, 1991·Clinical Immunology and Immunopathology·T ReichertJ Linden
Feb 13, 2001·British Journal of Haematology·J Kluin-NelemansUNKNOWN Dutch Co-operative Study Group on Immunophenotyping of Haematological Malignancies (SIHON)
May 1, 1993·The American Journal of Medicine·Gail Shor-PosnerMarianna K Baum
Dec 21, 2006·Cytometry. Part B, Clinical Cytometry·Bruno BrandoJan Willem Gratama
Sep 1, 1994·Journal of Clinical Immunology·M C AldhousK S Froebel
Feb 5, 2000·Occupational and Environmental Medicine·A D Curran
Jan 1, 1992·Cytometry·J P RobinsonG Durack
Oct 18, 2016·Transplantation·Oscar Kenneth SerranoTimothy L Pruett
Dec 24, 1997·Journal of Acquired Immune Deficiency Syndromes and Human Retrovirology : Official Publication of the International Retrovirology Association·M K BaumJ B Page
Aug 15, 1998·Journal of Acquired Immune Deficiency Syndromes and Human Retrovirology : Official Publication of the International Retrovirology Association·W K HootsC Contant
Apr 30, 1998·Journal of Acquired Immune Deficiency Syndromes and Human Retrovirology : Official Publication of the International Retrovirology Association·Z GrossmanN Intrator
Jun 1, 1997·Journal of Acquired Immune Deficiency Syndromes and Human Retrovirology : Official Publication of the International Retrovirology Association·E A OperskalskiD O Stram
Mar 9, 2000·Journal of Acquired Immune Deficiency Syndromes : JAIDS·G Shor-PosnerM K Baum
Jul 6, 2000·Revista Da Sociedade Brasileira De Medicina Tropical·J P MonteiroM L Bianchi
Oct 1, 1994·Clinical Microbiology Reviews·J J McSharry
May 1, 1996·Clinical and Diagnostic Laboratory Immunology·K M KamW P Mak
Aug 1, 1992·Clinical Immunology and Immunopathology·A E PlatonovL V Serebrovskaya

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

The Journal of Asthma : Official Journal of the Association for the Care of Asthma
Karin LisspersKurt Svärdsudd
Transfusion
Syria Laperche, Francophone African Group for Research in Blood Transfusion
© 2021 Meta ULC. All rights reserved