Leukocyte marker CD43 promotes cell growth in co-operation with β-catenin in non-hematopoietic cancer cells

International Journal of Oncology
A BalikovaL Kadaja-Saarepuu

Abstract

The Wnt/β-catenin pathway regulates key cellular processes such as differentiation, proliferation, apoptosis; and its activation promotes development of several cancer types. Expression of CD43 (leukosialin), the predominant leukocyte transmembrane sialoglycoprotein, has been detected in many tumors of non-hematopoietic origin. CD43 participates in cell adhesion and regulates intracellular signal transduction pathways involved in cell proliferation and survival. The cytoplasmic domain of CD43 has been reported to translocate to the nucleus, interact with β-catenin and affect its target gene expression, but the impact of this action on cell fate is still unknown. We demonstrate, here, by colony formation assay and siRNA-mediated gene silencing that CD43 and β-catenin co-operate in promoting cell growth. Moreover, in cells with down-regulated β-catenin expression the activation of p53 in response to CD43 overexpression is significantly impaired. In addition, the presence of both CD43 and β-catenin is required for the TCF/LEF-mediated transcription. Presumably, the full-length CD43 participates in this transcriptional regulation. We show that the mature CD43 localizes to the nucleus, where it binds chromatin, co-localizes and co-i...Continue Reading

Citations

Nov 22, 2013·PloS One·Nohemi Camacho-ConchaGustavo Pedraza-Alva
Dec 12, 2013·Journal of Cellular Physiology·A O Galindo-AlbarránM A Santana
Feb 13, 2017·The Journal of Experimental Medicine·Hilde SchjervenMarkus Müschen
Aug 23, 2020·International Journal of Molecular Sciences·Jinfen WeiHongli Du

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