Leukocyte populations and IL-6 in the tumor microenvironment of an orthotopic colorectal cancer model

Acta Biochimica Et Biophysica Sinica
Sarah MillerKulmira Nurgali

Abstract

Colorectal cancer (CRC) is a major health problem worldwide. It is often diagnosed late due to its asymptomatic nature. As with all cancers, an immune reaction is involved; however, in CRC, it is unknown if this immune response is favorable or unfavorable for disease progression. In this study, the immune response in mesenteric lymph nodes (MLNs) and Peyer's patches was investigated during development of CRC in an orthotopic mouse model. CRC was induced by injecting CT26 cells into the cecum wall of BALB/c mice. Flow cytometry was used to analyze leukocyte populations involved in tumor immunity in MLNs and Peyer's patches. Cryostat sections for immunohistochemistry were prepared from the caecum and colon from CRC-induced and sham-operated animals. Cytokines produced by mouse CT26 cell line were measuredin vitroandin vivo Significant increases in the number of CD8(+)/TCR(+)and CD49b(+)/TCR(-)(natural killer) cells were found in MLNs and Peyer's patches in the CRC group. In addition, γδT cells were present in the lamina propria of the colon tissues from sham-operated mice, but absent in the colon tissues from mice with CRC. Immunohistochemical analysis of tumorous tissues showed eosinophil, CD69(+)T cell, and CD11b(+)cell infiltr...Continue Reading

References

Mar 7, 1998·Cancer Immunology, Immunotherapy : CII·G A PieterszI F McKenzie
Mar 29, 2001·Nature Immunology·M J SmythJ A Trapani
Jul 29, 2003·Journal of Surgical Oncology·Yuan-Chang Chung, Ya-Fen Chang
Mar 30, 2004·Clinical Immunology : the Official Journal of the Clinical Immunology Society·Edward MorganAmitabh Gaur
May 4, 2004·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·M C Bibby
Apr 20, 2005·Digestive Surgery·M W HeijstekI H M Borel Rinkes
Aug 12, 2005·Cancer Immunology, Immunotherapy : CII·Graziella BelloneGiorgio Emanuelli
Oct 8, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·Murielle CorvaisierFrancine Jotereau
Mar 8, 2006·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·Antonio SicaPaola Allavena
Mar 4, 2008·Gastroenterology Clinics of North America·Mitchell S Cappell
Jun 21, 2008·The New England Journal of Medicine·Olivera J Finn
Oct 7, 2008·Oncogene·I Waldhauer, A Steinle
May 21, 2009·The Journal of Immunology : Official Journal of the American Association of Immunologists·Matilde TodaroGiorgio Stassi
Jul 7, 2009·Archivum Immunologiae Et Therapiae Experimentalis·Andrzej SzkaradkiewiczBarbara Zwoździak
Apr 28, 2010·Gastroenterology·C Richard Boland, Ajay Goel
Jun 12, 2010·Nature Reviews. Immunology·Marc BonnevilleWilli K Born
Nov 12, 2010·The Journal of Immunology : Official Journal of the American Association of Immunologists·Fanny LegrandMonique Capron
Dec 15, 2010·Nature Reviews. Immunology·Maya Saleh, Giorgio Trinchieri
Feb 8, 2011·Biochimica Et Biophysica Acta·Jürgen SchellerStefan Rose-John
Jun 1, 2011·BMC Gastroenterology·Barbara-Ann AdelsteinLes Irwig
Sep 13, 2011·Cancer Microenvironment : Official Journal of the International Cancer Microenvironment Society·Marco ErreniPaola Allavena
Sep 20, 2011·The Journal of Clinical Investigation·Daniel T FisherSharon S Evans
Jan 3, 2012·Seminars in Cancer Biology·Frances R Balkwill, Alberto Mantovani
Jun 21, 2012·Clinica Chimica Acta; International Journal of Clinical Chemistry·A-Jin LeeIm-Hee Shin
Dec 22, 2012·Blood·Bernhard Moser
Apr 30, 2014·Cancer Immunology Research·Benjamin P Davis, Marc E Rothenberg
Jul 18, 2015·Molecular Cancer Research : MCR·Saroor A A Patel, Nigel J Gooderham

❮ Previous
Next ❯

Citations

Jan 18, 2018·Journal of Experimental & Clinical Cancer Research : CR·Nyanbol KuolKulmira Nurgali
Sep 15, 2017·Oncotarget·Xianda ZhaoSubbaya Subramanian
Feb 10, 2021·International Journal of Molecular Sciences·Aldona Kasprzak
Feb 6, 2022·BMC Complementary Medicine and Therapies·Abilasha Gayani JayathilakeXiao Qun Su

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cancer Biology: Molecular Imaging

Molecular imaging enables noninvasive imaging of key molecules that are crucial to tumor biology. Discover the latest research in molecular imaging in cancer biology in this feed.