Abstract
The turn-over of leukocytes at sites of inflammation in vivo is to a large extent uninvestigated, mainly due to the technical difficulties associated with sampling and analysis of the inflammatory exudate. This paper investigates the immigration of fluorescently labeled granulocytes into exudate chambers at 8 h and at 1, 3, and 6 days after implantation into abdominal muscle of rat. In each experiment, the circulating granulocytes were labeled by intravenous administration of the DNA-labeling fluorochrome Hoechst 33342 and allowed to migrate into the chamber during 6 h before harvesting the chamber exudate. The rate of granulocyte immigration into the chamber varied considerably over time, showing a minimum at 3 days after implantation. The resulting kinetic pattern of granulocyte numbers in the exudate showed a two-step appearance, different from that of earlier determinations in soft tissue. A comparison between the calculated rates of granulocyte immigration and the total number of granulocytes present in the exudate at different times indicated that all immigrated cells survived in the chamber for the entire observation period of 6 days.
References
Nov 1, 1991·Biomaterials·A S Eriksson, P Thomsen
Feb 21, 1991·Nature·O SpertiniT F Tedder
Apr 1, 1990·Immunological Reviews·M PatarroyoC G Gahmberg
Jan 1, 1991·The Journal of Clinical Investigation·E Ruoslahti
Dec 1, 1987·The Journal of Clinical Investigation·C F Nathan
Jun 1, 1989·Agents and Actions·S Psychoyos, S Uziel-Fusi
Apr 21, 1989·Journal of Immunological Methods·D Loeffler, S Ratner
Sep 1, 1989·British Journal of Cancer·P L Olive
Dec 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·M P BevilacquaM A Gimbrone
Jan 1, 1988·Methods in Enzymology·D G Wright
Dec 1, 1988·The Journal of Clinical Investigation·M S LewisG A Zimmerman
Apr 1, 1988·ASAIO Transactions·J M Anderson
Jan 1, 1988·Biomaterials·A S ErikssonP Thomsen
Apr 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·S D WrightB W Erickson
Apr 1, 1972·The Journal of Physiology·A Atherton, G V Born
Oct 1, 1982·The Journal of Cell Biology·S T HoffsteinG Weissmann
Feb 1, 1993·The Journal of Clinical Investigation·M P Bevilacqua, R M Nelson
Aug 1, 1993·The Journal of Experimental Medicine·W A MullerD M Phillips
Jun 19, 1964·Science·A JANOFF, B W ZWEIFACH
Citations
Jan 1, 1996·Journal of Cellular Physiology·A S Eriksson, P Thomsen
Oct 1, 1996·Inflammation·A S Eriksson, R Sinclair
Jun 25, 1998·Journal of Homosexuality·J Liliequist