PMID: 8967378Apr 1, 1996Paper

Leukocyte rolling velocity and its relation to leukocyte-endothelium adhesion and cell deformability

The American Journal of Physiology
Herbert H LipowskyJ S Cartmell

Abstract

To explore the relationship between the rolling velocity of leukocytes (VWBC) and wall shear rates (gamma), measurements of VWBC were made along the length of rat mesenteric venules in which a gradient in gamma was induced by compressing the venule with a blunted microprobe to form a stenosis in which gamma varied from 300 to 1500 s-1. For individual WBCs that rolled through stenosis, VWBC was proportional to gamma for its entire range, in contrast to previous studies that have shown a plateau in VWBC vs. gamma for the ensemble population. Comparisons of the slope of VWBC/gamma for individual cells with ensemble values of VWBC obtained in the entrance region of the stenosis were made during suffusion of the tissue with the chemoattractant N-formylmethionyl-leucyl-phenylalanine (FMLP), to increase WBC-EC adhesion and WBC stiffness, or colchicine and cytochalasin B to increase WNC deformability. Under control conditions, the slope of individual cells was significantly 20% greater than VWBC/gamma, whereas it was significantly reduced by 48% during suffusion with FMLP. With exposure to colchicine, the slope was 78% lower than VWBC/gamma and compared with control was similar in magnitude to that obtained with FMLP. Cytochalasin also...Continue Reading

Citations

Mar 8, 2003·American Journal of Physiology. Heart and Circulatory Physiology·Katherine B Abbitt, Gerard B Nash
Nov 10, 2001·Annual Review of Biomedical Engineering·G W Schmid-Schönbein
Sep 29, 2000·American Journal of Physiology. Heart and Circulatory Physiology·M J Pearson, H H Lipowsky
May 19, 2007·American Journal of Physiology. Gastrointestinal and Liver Physiology·Emile RijckenChristian F Krieglstein
Jul 13, 2006·Annual Review of Biomedical Engineering·Geert W Schmid-Schönbein
Dec 15, 2010·American Journal of Physiology. Heart and Circulatory Physiology·Herbert H LipowskyAnne Lescanic
Aug 28, 2004·American Journal of Physiology. Heart and Circulatory Physiology·Michael B Kim, Ingrid H Sarelius

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