Leukocytospermia and sperm preparation--a flow cytometric study.

Reproductive Biology and Endocrinology : RB&E
Giuseppe RicciG Presani

Abstract

Leukocytes represent the predominant source of reactive oxygen species both in seminal plasma and in sperm suspensions and have been demonstrated to negatively influence sperm function and fertilization rate in assisted reproduction procedures. Peroxidase test is the standard method recommended by WHO to detect semen leukocytes but it may be inaccurate. The aims of this study were (i) to compare the efficiency of swim-up and density-gradient centrifugation techniques in removing seminal leukocytes, (ii) to examine the effect of leukocytes on sperm preparation, and (iii) to compare flow cytometry and peroxidase test in determining leukocyte concentration in semen using a multiparameter flow cytometric method. Semen samples from 126 male partners of couples undergoing infertility investigations were analyzed for leukocytospermia using standard optical microscopy and flow cytometry. Sixty-nine out of 126 samples were also processed using simultaneously the swim-up and density-gradient centrifugation techniques. A multiparameter flow cytometric analysis to assess simultaneously sperm concentration, sperm viability, sperm apoptosis, and leukocyte concentration was carried out on neat and prepared sperm. Both sperm preparation method...Continue Reading

References

Mar 1, 1992·Journal of Reproduction and Fertility·E KessopoulouI D Cooke
Dec 3, 1999·Human Reproduction Update·F R Ochsendorf
Jun 1, 2000·Human Reproduction·G RicciS Perticarari
Oct 25, 2002·Molecular Human Reproduction·Shun-Long WengSergio Oehninger
May 17, 2003·Fertility and Sterility·Ashok AgarwalMohamed A Bedaiwy
Oct 11, 2003·Andrologia·R HenkelW-B Schill
Nov 18, 2003·Reproductive Biology and Endocrinology : RB&E·Ralf R Henkel, Wolf-Bernhard Schill
Jun 26, 2004·Human Reproduction Update·W C L Ford
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Feb 19, 2008·Human Reproduction Update·Kelton Tremellen
Feb 3, 2009·Journal of Assisted Reproduction and Genetics·Roberta Maria FarielloAgnaldo Pereira Cedenho

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Citations

Dec 18, 2012·Current HIV/AIDS Reports·Anupriya AggarwalStuart G Turville
Jun 19, 2012·Reproductive Biology and Endocrinology : RB&E·Mario CavagnaJosé G Franco
Nov 22, 2014·The Journal of Infectious Diseases·Joseph A PolitchDeborah J Anderson
Oct 6, 2015·BioMed Research International·Giuseppe RicciElisa Trevisan
May 2, 2012·Systems Biology in Reproductive Medicine·Abbas Kiani-EsfahaniMohammad H Nasr-Esfahani
May 6, 2015·Fertility and Sterility·Giuseppe RicciMassimo Borelli
May 1, 2015·Reproductive Biology and Endocrinology : RB&E·Stefania LuppiGiuseppe Ricci
May 12, 2018·Human Fertility : Journal of the British Fertility Society·Akram HosseiniNahid Yari
Feb 4, 2019·Journal of Assisted Reproduction and Genetics·Luisa ZupinGiuseppe Ricci

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Methods Mentioned

BETA
density-gradient centrifugation
flow cytometry
density-gradient
Density gradient centrifugation
Optical
gradient density-centrifugation

Software Mentioned

CELLQuest Pro
GraphPad Prism
Statistica
GraphPad

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