Leukotriene D4 enhances immunoglobulin production in CD40-activated human B lymphocytes

The Journal of Allergy and Clinical Immunology
Josée LamoureuxMarek Rola-Pleszczynski

Abstract

Cysteinyl-leukotrienes (cysLTs) are lipid mediators recognized for their role in asthma and allergic inflammation. CysLT1, one of the receptors for cysLTs, is expressed, among others, in bronchial smooth muscle cells, phagocytes, and B lymphocytes. The potential role of CysLT1 in B lymphocytes remains unexplored. We examined the modulation of expression and function of CysLT1 in human B lymphocytes. Human B lymphocytes were purified from peripheral blood and analyzed by means of RT-PCR and flow cytometry, after culture with IL-4 and anti-CD40 antibody or CD154-transfected fibroblasts. Functional responses to leukotriene (LT) D4 in terms of cytosolic calcium flux, proliferation, and immunoglobulin production were also examined. B lymphocytes expressed CysLT1 at both the mRNA and protein levels. Two-fold to 3-fold enhancement of CysLT1 expression was observed after B-cell exposure to a combination of activating anti-CD40 antibody and IL-4. The expression of CysLT1 was also enhanced when B lymphocytes were cocultured with CD154-transfected fibroblasts in the presence of IL-4. Moreover, IL-4 and CD40-activated B lymphocytes showed an increased responsiveness to LTD4 in terms of cytosolic calcium flux, which was totally prevented by...Continue Reading

Citations

Oct 19, 2007·Cancer Metastasis Reviews·Graham P PidgeonKenneth V Honn
Jul 9, 2008·Current Allergy and Asthma Reports·Marc Peters-Golden
Jun 6, 2012·Mediators of Inflammation·Mary K McCarthy, Jason B Weinberg
Jul 1, 2006·American Journal of Respiratory Cell and Molecular Biology·Charles ThompsonMarek Rola-Pleszczynski
Jun 3, 2014·American Journal of Respiratory Cell and Molecular Biology·Cynthia KanagarathamDanuta Radzioch
Feb 13, 2016·Prostaglandins, Leukotrienes, and Essential Fatty Acids·Louiza YaddadenJana Stankova
Mar 1, 2011·Biochimica Et Biophysica Acta·Katsuhide Okunishi, Marc Peters-Golden
Jan 1, 2008·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·A S KaposiA Treszl
Mar 20, 2013·Pediatric Allergy and Immunology : Official Publication of the European Society of Pediatric Allergy and Immunology·Maria A ZamboninoJose L Corzo
Dec 16, 2010·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·I DoñaG Canto
Jun 26, 2007·The Journal of Allergy and Clinical Immunology·Javier Chinen, William T Shearer
May 22, 2013·The Journal of Allergy and Clinical Immunology·Taylor A DohertyDavid H Broide
Aug 14, 2003·Toxicology and Applied Pharmacology·X Huang, Q Zhang
Nov 21, 2016·Allergology International : Official Journal of the Japanese Society of Allergology·Tuğba ArikogluSemanur Kuyucu
Sep 21, 2018·Frontiers in Immunology·Nincy Debeuf, Bart N Lambrecht
Jun 25, 2010·The Journal of Immunology : Official Journal of the American Association of Immunologists·Akiko MaekawaYoshihide Kanaoka
Aug 25, 2018·Proceedings of the National Academy of Sciences of the United States of America·Jamie L LahvicLeonard I Zon

❮ Previous
Next ❯

Related Concepts

Related Feeds

B cells: Gene Expression

B lymphocytes are white blood cells that play a role in the adaptive immune system by secreting antibodies. Here is the latest research on gene expression in B cells.

Allergy and Asthma

Allergy and asthma are inflammatory disorders that are triggered by the activation of an allergen-specific regulatory t cell. These t cells become activated when allergens are recognized by allergen-presenting cells. Here is the latest research on allergy and asthma.

Calcium & Bioenergetics

Bioenergetic processes, including cellular respiration and photosynthesis, concern the transformation of energy by cells. Here is the latest research on the role of calcium in bioenergetics.