PMID: 9002425Jan 1, 1997Paper

Leukotriene D4-induced Ca2+ mobilization in Ehrlich ascites tumor cells

The Journal of Membrane Biology
S F PedersenI H Lambert

Abstract

Stimulation of Ehrlich ascites tumor cells with leukotriene D4 (LTD4) within the concentration range 1-100 nm leads to a concentration-dependent, transient increase in the intracellular, free Ca2+ concentration, [Ca2+]i. The Ca2+ peak time, i.e., the time between addition of LTD4 and the highest measured [Ca2+]i value, is in the range 0.20 to 0.21 min in ten out of fourteen independent experiments. After addition of a saturating concentration of LTD4 (100 nm), the highest measured increase in [Ca2+]i in Ehrlich cells suspended in Ca2+-containing medium is 260 +/- 14 nm and the EC50 value for LTD4-induced Ca2+ mobilization is estimated at 10 nM. Neither the peptido-leukotrienes LTC4 and LTE4 nor LTB4 are able to mimic or block the LTD4-induced Ca2+ mobilization, hence the receptor is specific for LTD4. Removal of Ca2+ from the experimental buffer significantly reduces the size of the LTD4-induced increase in [Ca2+]i. Furthermore, depletion of the intracellular Ins(1,4,5)P3-sensitive Ca2+ stores by addition of the ER-Ca2+-ATPase inhibitor thapsigargin also reduces the size of the LTD4-induced increase in [Ca2+]i in Ehrlich cells suspended in Ca2+-containing medium, and completely abolishes the LTD4-induced increase in [Ca2+]i in ...Continue Reading

Citations

Dec 11, 1997·The Journal of Experimental Zoology·E K Hoffmann
May 9, 2001·Biochimica Et Biophysica Acta·J MollerupE K Hoffmann
Mar 27, 2002·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·E K Hoffmann, C Hougaard
Jan 8, 2009·Physiological Reviews·Else K HoffmannStine F Pedersen
Jan 18, 2003·American Journal of Physiology. Heart and Circulatory Physiology·Pinggang LiuVenkat Gopalakrishnan
Jan 14, 1999·The American Journal of Physiology·N K JorgensenE K Hoffmann
Nov 14, 1998·Biochimica Et Biophysica Acta·S F PedersenE K Hoffmann

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