Abstract
In an attempt to elucidate the effect of lipoxygenase inhibitors on hepatic injury, we investigated D-galactosamine (GalN)-treated C57BL/6 mice receiving an intravenous (i.v.) injection of lipopolysaccharide (LPS)-activated autologous spleen cells. As compared with control spleen cells, the number of monocytes in the spleen cells isolated from LPS-treated mice and their oxidative free radical production increased markedly. Oxygen radical production by the dish-adherent cells (macrophage-rich population) was enhanced a further 4-fold. Although hepatotoxicity was not demonstrated in mice treated with 20 mg GalN alone, marked hepatic injury was found in the GalN-treated mice with a supplementation of LPS-activated spleen cells. The dish-adherent cells aggravated this hepatic injury, in contrast to minor hepatotoxicity by the nonadherent cells. Oxygen radical production by LPS-activated spleen cells was markedly reduced by the lipoxygenase inhibitors (azelastine, ketotifen and AA861). Hepatotoxicity was scarcely detected in the GalN-treated mice with a supplementation of the LPS-activated spleen cells which had been previously treated with lipoxygenase inhibitors. From these results, LPS-activated spleen macrophages contributed to ...Continue Reading
References
Nov 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·C GalanosW Reutter
Jul 1, 1988·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Y ShiratoriK Kamii
Feb 1, 1985·Experimental and Molecular Pathology·T A Tran-ThiK Decker
Jul 1, 1987·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·W HagmannD Keppler
Mar 1, 1987·The Journal of Experimental Medicine·V LehmannC Galanos
Sep 1, 1985·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·D KepplerH K Koch
May 1, 1985·Seminars in Liver Disease·K Decker
Jul 21, 1971·Nature: New Biology·P Alexander, R Evans
Dec 1, 1983·Prostaglandins·Y AshidaY Maki
Jul 16, 1984·European Journal of Biochemistry·M Birmelin, K Decker
Jul 7, 1982·Molecular and Cellular Biochemistry·J MandlT Garzó
Sep 1, 1980·Annals of Internal Medicine·S J Klebanoff
Apr 5, 1983·European Journal of Biochemistry·M Bermelin, K Decker
May 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·G A HiggsJ R Vane
Feb 1, 1983·Proceedings of the Society for Experimental Biology and Medicine·D S CamaraJ P Nolan
Aug 1, 1984·The Journal of Clinical Investigation·S L KunkelG I Higashi
Apr 1, 1984·The Journal of Infectious Diseases·J P GutA Kirn
Aug 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·C A RouzerJ M Manning
Nov 1, 1982·The Journal of Infectious Diseases·J P GutA Kirn
Mar 1, 1981·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·A Al-TuwaijriN R Di Luzio
Citations
Jul 1, 1994·Digestive Diseases and Sciences·Y ShiratoriT Kawase
Oct 1, 1996·Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.]·H M OmarJ B Watkins
Jan 1, 1993·Pharmacology & Therapeutics·J Quiroga, J Prieto
Jul 7, 2000·Life Sciences·T X CuiM Horiuchi
Apr 24, 2001·Free Radical Biology & Medicine·N WatanabeH Ishii
Aug 4, 2009·Chemical Research in Toxicology·Debra L Laskin
Jan 6, 2011·The Journal of Clinical Investigation·Carlos H SerezaniMarc Peters-Golden
Oct 5, 2010·Annual Review of Pharmacology and Toxicology·Debra L LaskinJeffrey D Laskin
Apr 1, 1995·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·D L LaskinD J Jollow
Jan 1, 1996·Toxicologic Pathology·D L Laskin