Abstract
2,5-Hexanedione (2,5-HD) exposure in the rat produces irreversible testicular atrophy, a model of human male infertility that can be used for mechanistic and therapeutic studies. Following testicular injury by 2,5-HD, stem cell factor (SCF), a Sertoli cell-derived growth factor that binds the c-kit receptor on spermatogonia, is altered in its expression, changing from predominantly membrane SCF to predominantly soluble SCF. The goals of this study were 2-fold: first, evaluate leuprolide, a GnRH agonist, as a therapy for 2,5-HD-induced testicular atrophy, and second, examine changes in SCF expression during testicular injury and following recovery from injury. Rats exposed to 2,5-HD showed a nearly complete testicular atrophy that could be reversed by leuprolide therapy. Using RT-PCR, preferential expression of membrane SCF was associated with spermatogenesis, whereas soluble SCF expression was associated with atrophy. In conclusion, 2,5-HD exposure altered the form of SCF expressed and disrupted spermatogenesis; leuprolide therapy allowed recovery of spermatogenesis, which correlated with a normalization in growth factor expression in an otherwise irreversibly atrophic testis.
References
Jan 1, 1977·Molecular and Cellular Endocrinology·J S ElkingtonE Steinberger
Jan 1, 1992·The Journal of Urology·D A DiamondL Seethalakshmi
Oct 1, 1992·Genes & Development·C I BrannanN G Copeland
Sep 12, 1992·BMJ : British Medical Journal·E CarlsenN E Skakkebaek
Aug 1, 1992·The Anatomical Record·M P McGuinness, J M Orth
Oct 5, 1990·Cell·D M AndersonD Cosman
Mar 8, 1991·Cell·J G FlanaganP Leder
Aug 29, 1991·Nature·S DolciP J Donovan
Dec 1, 1991·Toxicology and Applied Pharmacology·K J JohnsonK Boekelheide
Oct 5, 1990·Cell·F H MartinE A Mendiaz
Jan 1, 1988·Toxicology and Applied Pharmacology·K Boekelheide
Jan 1, 1988·Toxicology and Applied Pharmacology·K Boekelheide
Jan 1, 1981·Endocrine Reviews·A J Hsueh, P B Jones
Jan 1, 1984·Toxicologic Pathology·R E ChapinJ C Lamb
Jan 1, 1983·Australian Journal of Biological Sciences·B P Setchell, K A Galil
Apr 1, 1983·Experimental and Molecular Pathology·R E ChapinJ S Bus
Mar 15, 1980·Toxicology and Applied Pharmacology·W J KrasavageC J Terhaar
Apr 1, 1980·Toxicology and Applied Pharmacology·L PerbelliniI Pavan
May 15, 1994·Archives of Biochemistry and Biophysics·K E LangleyH S Lu
Aug 1, 1995·Endocrinology·M KangasniemiM L Meistrich
Jul 1, 1995·Biology of Reproduction·E K AllardK Boekelheide
May 1, 1993·Developmental Biology·G MarzialiV Sorrentino
May 1, 1993·Developmental Biology·K ManovaR F Bachvarova
Jul 1, 1995·Endocrinology·M KangasniemiM L Meistrich
Feb 2, 1995·The New England Journal of Medicine·J AugerP Jouannet
Feb 2, 1995·The New England Journal of Medicine·S S Howards
Feb 2, 1995·The New England Journal of Medicine·R J Sherins
Oct 1, 1994·Toxicology and Applied Pharmacology·J H RichburgK Boekelheide
Aug 1, 1994·Pharmaceutical Research·H OkadaH Toguchi
Jan 1, 1994·Reproduction, Fertility, and Development·D M de Kretser
May 1, 1994·The Journal of Urology·P B HudsonM E Lombardo
Sep 1, 1996·Endocrinology·M L MeistrichI Huhtaniemi
Jul 1, 1996·Biology of Reproduction·E K AllardK Boekelheide
Apr 1, 1996·Toxicology and Applied Pharmacology·E K Allard, K Boekelheide
Dec 1, 1996·Endocrinology·W E TaylorS Bhasin
Citations
Jun 3, 2008·Reproduction : the Official Journal of the Society for the Study of Fertility·Marvin L Meistrich, Gunapala Shetty
Dec 3, 2014·Progress in Histochemistry and Cytochemistry·Marília I FigueiraSílvia Socorro
Jan 5, 2008·Endocrinology·Sailaja KoduriBarbara J Attardi
Dec 6, 2001·Protoplasma·P VecinoJ Aréchaga
Mar 27, 2004·Biology of Reproduction·Sheri Ann HildJerry R Reel
Feb 28, 2003·Biology of Reproduction·Masako OhmuraHajime Sawada
Sep 25, 2002·Toxicology Letters·John H RichburgDavid J Dix
Apr 11, 2008·The Journal of Steroid Biochemistry and Molecular Biology·Nikolaos SofikitisNikolaos Pardalidis
Mar 19, 2016·Andrology·G ShettyM L Meistrich
Nov 15, 2000·Developmental Biology·W YanJ Toppari
Jul 23, 2003·Developmental Biology·Anita S-R PepperE Jane Albert Hubbard
Dec 28, 1999·Tissue & Cell·T OgawaR L Brinster
Nov 22, 2001·Biology of Reproduction·S A HildR P Blye
Aug 24, 1999·Biology of Reproduction·D G de RooijY Nishimune
Jun 23, 2001·Biology of Reproduction·S A HildJ R Reel
Mar 22, 2001·Biology of Reproduction·H A SchoenfeldK Boekelheide
Feb 24, 2001·Toxicologic Pathology·D M Creasy
Apr 9, 2005·Human Reproduction Update·N SofikitisI Miyagawa
May 16, 2008·Journal of Huazhong University of Science and Technology. Medical Sciences = Hua Zhong Ke Ji Da Xue Xue Bao. Yi Xue Ying De Wen Ban = Huazhong Keji Daxue Xuebao. Yixue Yingdewen Ban·Yue Qian, Fuqing Zeng
Mar 6, 1999·Endocrinology·W YanJ Toppari
Apr 17, 2002·Endocrinology·Ans M M van PeltFederica M F van Dissel-Emiliani
May 10, 2000·Endocrinology·G ShettyM L Meistrich
Apr 22, 2006·Endocrinology·Gunapala ShettyMarvin L Meistrich
Oct 19, 2017·Stem Cell Research & Therapy·Shadan NavidYumi Hoshino
Aug 16, 2005·Journal of Animal Science·H Jiménez-SeverianoJ E Kinder
Mar 14, 2003·Journal of Andrology·Marvin L Meistrich, Gunapala Shetty
Feb 17, 2005·Journal of Andrology·Kim BoekelheideMarvin L Meistrich
Aug 22, 2002·Toxicologic Pathology·Lynda L LanningDawn G Goodman
May 20, 2004·Reproductive Medicine and Biology·Kazuo AokiMasanobu Kudo
Feb 18, 2003·International Journal of Urology : Official Journal of the Japanese Urological Association·Fumiyasu EndoHideyuki Akaza
Jun 8, 2001·Tissue & Cell·I DobrinskiR L Brinster
Jan 1, 2000·The American Journal of the Medical Sciences·R I HenkinR P Agarwal
Dec 5, 1998·Tissue & Cell·T OgawaR L Brinster