Abstract
Autoantibodies of the cryoprecipitating IgG3 isotype have been shown to play a significant role in the development of murine lupus-like autoimmune syndrome. At present, the structural basis of IgG3 cryoprecipitation and its role in autoantibody pathogenicity remain to be defined. Using molecular variants of an IgG3 monoclonal rheumatoid factor, 6-19, derived from an autoimmune MRL-Fas(lpr) mouse, we have investigated the implication of charged residues in the heavy-chain variable (VH) region, potential CH3-linked oligosaccharides, and galactosylation of CH2-linked oligosaccharides in its cryoglobulin activity. The cryoglobulin activity of the IgG3 6-19 mutant bearing more negatively charged residues at VH 6 and 23 was found to be reduced but still highly significant, whereas that of the mutant lacking a potential CH3 glycosylation site remained unchanged. In marked contrast, IgG3 6-19 variants obtained from 6-19 heavy-chain transgenic mice displayed barely detectable cryoglobulin activity associated with an increased level of galactosylation in the CH2 oligosaccharide side chains. Thus, our data strongly suggest that the cryoglobulin activity of IgG3 6-19 autoantibody is critically determined by levels of galactosylation in the...Continue Reading
References
Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·F SangerA R Coulson
Jan 1, 1975·Immunochemistry·P H Saulk, W Clem
Jan 1, 1992·International Immunology·T BerneyS Izui
Jan 1, 1992·Kidney International·R LemoineS Izui
Feb 1, 1991·The Journal of Experimental Medicine·R ShefnerB Diamond
Jan 1, 1990·The Journal of Experimental Medicine·M ShlomchikM Weigert
Dec 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·L ReiningerS Izui
Feb 1, 1989·European Journal of Immunology·F SpertiniS Izui
Apr 1, 1989·Journal of Autoimmunity·R ParekhT Rademacher
May 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·R OrlandiG Winter
Aug 2, 1987·Nature·M J ShlomchikM G Weigert
Aug 1, 1985·Nature·R B ParekhK Matsuta
Nov 1, 1974·The American Journal of Medicine·J C BrouetM Seligmann
Aug 1, 1968·The Journal of Clinical Investigation·H M GreyE C Franklin
Feb 1, 1971·The Journal of Experimental Medicine·H M GreyM Cohn
Mar 1, 1970·The Journal of Clinical Investigation·J D Capra, H G Kunkel
Jan 1, 1984·Journal of Biochemistry·Y Nishimura, H Nakamura
Jan 1, 1995·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·J LundR Jefferis
Jan 1, 1995·European Journal of Immunology·D J PankaA Marshak-Rothstein
Feb 1, 1993·Molecular Immunology·S TakahashiM Kyogoku
Mar 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·T FulpiusS Izui
Apr 1, 1996·The Journal of Clinical Investigation·P C SwansonG D Glick
Jun 1, 1997·Molecular Immunology·D J Panka
Dec 8, 1998·European Journal of Immunology·F JovelinS Koutouzov
Jan 9, 1999·Glycoconjugate Journal·K ShikataT Mizuochi
Mar 28, 2001·Science·P M RuddR A Dwek
May 23, 2001·Blood·T MizuochiS Izui
Jul 5, 2001·Carbohydrate Research·Y ShimizuT Mizuochi
Citations
Sep 6, 2006·Springer Seminars in Immunopathology·Lucie BaudinoShozo Izui
Jun 9, 2006·Bulletin of Experimental Biology and Medicine·N A Konstantinova, E N Karandashov
Apr 7, 2007·Bulletin of Experimental Biology and Medicine·E N KarandashovA N Komarov
Oct 22, 2013·La Revue de médecine interne·R GoulabchandP Guilpain
Apr 15, 2008·Current Opinion in Nephrology and Hypertension·Charles E Alpers, Kelly D Smith
Oct 2, 2012·Journal of the American Society of Nephrology : JASN·Masako OtaniShozo Izui
May 11, 2010·Journal of Periodontology·Gavrilo BrajovićNadezda Milosević-Jovcić
Jan 6, 2006·Kidney International·S H NasrV D D'Agati
Oct 3, 2006·Biotechnology and Bioengineering·F A A MajidM Al-Rubeai
Oct 16, 2004·Molecular Immunology·Hirokazu YagiKoichi Kato
Sep 28, 2015·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·Jonathan Maurice ChemounyRenato C Monteiro
Mar 25, 2014·Autoimmunity Reviews·Radjiv GoulabchandPhilippe Guilpain
Aug 24, 2006·Blood·Pierre BobéMounira K Chelbi-Alix
Feb 24, 2007·Blood·Lucie BaudinoShozo Izui
Jul 9, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·Yasuhiro KurodaShozo Izui
Feb 15, 2002·Pathology International·Y KurodaT Mizuochi
Jun 8, 2018·Frontiers in Immunology·Tomasz Klaus, Joanna Bereta
Jan 23, 2008·The Journal of Immunology : Official Journal of the American Association of Immunologists·Lucie BaudinoShozo Izui
Oct 23, 2008·The Journal of Immunology : Official Journal of the American Association of Immunologists·Lucie BaudinoShozo Izui
Oct 18, 2005·La Presse médicale·P GuilpainL Mouthon