Levels of antibody to defined antigens of Mycobacterium tuberculosis in tuberculous meningitis.

Journal of Clinical Microbiology
A ChandramukiJ Ivanyi

Abstract

The aim of the study was to develop an antibody immunoassay for tuberculous meningitis with a sensitivity greater than that of the initial bacterial smear while maintaining a 100% specificity. Antibody titers to five purified antigens of Mycobacterium tuberculosis were measured in cerebrospinal fluid by using an enzyme-linked immunosorbent assay technique. Seventy-four patients with tuberculous meningitis (26 culture positive) were compared with 26 patients with purulent meningitis, 69 patients with suspected but excluded tuberculous meningitis, and 29 patients with other neurological diseases. Antibody responses to both the 14-kilodalton (kDa) antigen and lipoarabinomannan were immunodominant, accounting for 74% of those with any antibody titer to a soluble extract of M. tuberculosis. A sensitivity of 61% was achieved (77% for the culture-positive samples) by using immunoglobulin G titers to lipoarabinomannan and the 14-, 19-, and 38-kDa antigens. Thus, estimation of levels of antibody in cerebrospinal fluid to selected mycobacterial antigens would be valuable in the diagnosis of tuberculous meningitis. The possible reasons for the immunodominance of the 14-kDa antigen and lipoarabinomannan in the pathogenesis of tuberculous m...Continue Reading

References

Oct 1, 1988·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·G BothamleyJ Ivanyi
Nov 1, 1988·Journal of Clinical Microbiology·P S JackettJ Ivanyi
May 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·R A YoungR W Davis
Apr 1, 1987·The Journal of Infectious Diseases·T M Daniel
Nov 1, 1987·The Journal of Infectious Diseases·W R LevisP Fried
Jun 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·D YoungR A Young
Mar 1, 1985·The Medical Clinics of North America·A Molavi, J L LeFrock
Nov 1, 1971·Journal of the Neurological Sciences·P M UdaniD K Dastur
Jul 4, 1981·Lancet·S SwartP A Millac
Dec 1, 1980·The Journal of Infectious Diseases·K E Powell, L S Farer
Jan 1, 1987·Immunology Today·D B YoungJ R Lamb

❮ Previous
Next ❯

Citations

Sep 1, 1992·Italian Journal of Neurological Sciences·M Macucci, D Sità
Oct 1, 1995·Clinical and Experimental Immunology·G FrisciaJ Ivanyi
Apr 1, 1997·Acta Neurologica Scandinavica·K P NairV Shanmugam
Aug 26, 2003·Journal of Clinical Laboratory Analysis·Sanjay K GargPrakash S Bisen
Apr 11, 2008·Clinical Microbiology Reviews·R Bryan RockPhillip K Peterson
Apr 10, 2014·Frontiers in Immunology·Juraj Ivanyi
Sep 1, 1992·Clinical and Experimental Immunology·A VerbonA H Kolk
Jun 1, 1994·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·L F López-CortésJ Pachón-Diaz
Oct 14, 2011·Clinical and Vaccine Immunology : CVI·Walid Ben-SelmaJalel Boukadida
Jul 20, 2007·Scandinavian Journal of Immunology·F AbebeG Bjune
Sep 15, 2010·Biomarker Insights·Rajpal S KashyapHatim F Daginawala
Dec 1, 2011·Microbiology and Molecular Biology Reviews : MMBR·Daniel J BretlThomas C Zahrt
Jan 1, 1997·Indian Journal of Pediatrics·S Mahadevan
Apr 4, 2018·The Journal of Immunology : Official Journal of the American Association of Immunologists·Alok ChoudharyAbraham Pinter
Apr 3, 2021·Frontiers in Immunology·Graham H Bothamley
Sep 21, 2011·Tuberculosis·Juraj Ivanyi

❮ Previous
Next ❯